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Nramp1 Gene Polymorphisms and Cutaneous Leishmaniasis: An Evaluation on Host Susceptibility and Treatment Outcome Pubmed



Fattahidolatabadi M1 ; Mousavi T1, 2 ; Mohammadibarzelighi H3 ; Irian S4 ; Bakhshi B5 ; Nilforoushzadeh MA6, 7 ; Shiranibidabadi L6, 8 ; Hariri MM9, 10 ; Ansari N6 ; Akbari N9, 10
Authors
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Authors Affiliations
  1. 1. Antimicrobial Resistance Research Center, Faculty of Medicine, Iran University of Medical Sciences, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Immunology, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Bacteriology and Virology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
  5. 5. Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  6. 6. Skin Diseases and Leishmaniasis Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  7. 7. Skin and Stem Cell Research Center, Tehran University of Medical Sciences, Tehran, Iran
  8. 8. Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  9. 9. Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Isfahan, Iran
  10. 10. Regional Educational Blood Transfusion Center, Isfahan, Iran

Source: Journal of Vector Borne Diseases Published:2016


Abstract

Background & objectives: Association between polymorphisms in the natural resistance associated macrophage protein 1 (NRAMP1) gene and susceptibility to cutaneous leishmaniasis (CL) has been demonstrated worldwide; however, the reported results were inconsistent. This study aimed to determine the association of NRAMP1 variants with susceptibility to CL infection and patients’ response to treatment in Isfahan province of Iran. Methods: Peripheral blood samples were collected from 150 patients with CL and 136 healthy controls. The CL patients were treated with intralesional injection of meglumine antimoniate. The polymorphic variants at NRAMP1 (A318V and D543N) were analyzed using PCR-RFLP. The chi-square test and Fisher’s exact test were used to compare frequencies of alleles and genotypes of polymorphisms between patient and healthy control populations. Results: There was a statistically significant difference in the D543N (rs17235409) polymorphism between the CL patients and healthy controls (p=0.008). However, no significant association was detected for A318V (rs201565523) polymorphism between groups (p=0.26). In addition, there was a lack of association between D543N and A318V genotypes with response to treatment (p=0.54 and p=0.31, respectively). Interpretation & conclusion: The results indicated that genetic variations of D543N (rs17235409) might be associated with susceptibility to CL infection. These data may be used for detection of sensitive individuals and prevention of CL in endemic areas. © 2016, Malaria Research Center. All rights reserved.