Pcr-Based Identification of Integrons Types and Extended-Spectrum Α-Lactamase Genes in Salmonella Species Collected From Pediatric Diarrheal Samples

Pcr-Based Identification of Integrons Types and Extended-Spectrum Α-Lactamase Genes in Salmonella Species Collected From Pediatric Diarrheal Samples Publisher

Eshaghi H¹ ; Alhooei S^{2, 3} ; Abbasi A⁴ ; Khodabandeh M¹ ; Amiri FH⁵ ; Pournajaf A⁶ ; Kiani M⁷ ; Gholami M⁸ ; Hendizade P⁵ ; Mohammadi M⁹

Show Affiliations

1. Department of Infectious Diseases, Pediatric's Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Internal Medicine, School of Medicine, Babol University of Medical Sciences, Babol, Iran
3. Clinical Research Development Unite of Rouhani Hospital, Babol University of Medical Sciences, Babol, Iran
4. Pediatric Chronic Kidney Disease Research Center, Children's hospital Medical Center, Tehran University of Medical Sciences, Tehran, Iran
5. Department of Microbiology, School of Medicine, Babol University of Medical Sciences, Babol, Iran
6. Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran
7. Department of microbiology, faculty of medicine, Shahid Sadoughi University of medical sciences, Yazd, Iran
8. Department of Microbiology and virology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
9. Noncommunicable Pediatric Diseases Research Center, Health Research institute, Babol University of Medical Sciences, Babol, Iran

Source: Gazi Medical Journal Published:2021

Abstract

Objective: Salmonella is a significant pathogen between food-borne diseases. Salmonella spp. strains that produce extended-spectrum β-Lactamases (ESBL) have become a medical problem for both antimicrobial therapy and infection control programs. The aim of this project was detection of ESBL genes and class I, II and III Integrons in the Salmonella isolates. Methods: 405 non-duplicative stool samples were obtained. Antibacterial susceptibility was defined by the disk diffusion and also double disk synergy test (DDST) was used for confirming of ESBL phenotype. The multiplex-PCRs were directed for recognition of ESBLs (TEM, CTX-M and SHV) and int (I, II, III) genes. Results: Out of 405 samples, 54 (13.4%) Salmonella were obtained. The highest resistance rate was related to the NA (51.8%), followed by SXT (50%), CTX (46.3%), and AMP (33.3%). DDST was conducted for all isolates and 7 (12.9%) Salmonella spp were ESBL positive. Molecular analysis showed that 5 (9.3 %) of isolates were carried blaTEM-1 which belonged to the S. infantis and S. typhimurium. Three (5.5%) non-typeable isolates and 2 S. typhimurium were positive for the CTX-M gene. The prevalence of different classes of integrons showed that 23 (42.5%) isolates carried the integrase (int) gene. Conclusion:This research demonstrates the predominant existence in the Salmonella of the TEM and CTX-M genes. So, class I integron were more prevalent than class II and III in Salmonella isolates. They are capable of transferring to bacteria of this genus and also the other genus of intestinal ones. ©Copyright 2021 by Gazi University Medical Faculty - Available on-line at web site http://medicaljournal.gazi.edu.tr/

2. High Efficacy of Molecular Methods for Identification of Salmonella Enterica Serogroups: A 2-Year Surveillance, Infectious Diseases in Clinical Practice (2015)

3. Evaluation of Class 1 and 2 Integrons and Antibiotic Resistance Pattern in Salmonella Enterica Isolated From Diarrheal Food-Borne Outbreaks in Iran, Journal of Food Quality and Hazards Control (2019)

4. Biofilm Formation, Antimicrobial Resistance Genes, and Genetic Diversity of Salmonella Enterica Subspecies Enterica Serotype Enteritidis Isolated From Food and Animal Sources in Iran, Journal of Global Antimicrobial Resistance (2023)

5. High Frequency of Class 1 Integrons in Escherichia Coli Isolated From Patients With Urinary Tract Infections in Yasuj, Iran, Iranian Red Crescent Medical Journal (2016)

6. Analysis of Resistance to Macrolide-Lincosamide-Streptogramin B Among Meca-Positive Staphylococcus Aureus Isolates, Osong Public Health and Research Perspectives (2019)

7. Antimicrobial Resistance Patterns, Virulence Gene Profiles, and Genetic Diversity of Salmonella Enterica Serotype Enteritidis Isolated From Patients With Gastroenteritis in Various Iranian Cities, Iranian Journal of Basic Medical Sciences (2021)

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Style	Citing Format
MLA	Eshaghi H, et al.. "Pcr-Based Identification of Integrons Types and Extended-Spectrum Α-Lactamase Genes in Salmonella Species Collected From Pediatric Diarrheal Samples." Gazi Medical Journal, vol. 32, no. 3, 2021, pp. 413-416.
APA	Eshaghi H, Alhooei S, Abbasi A, Khodabandeh M, Amiri FH, Pournajaf A, Kiani M, Gholami M, Hendizade P, Mohammadi M (2021). Pcr-Based Identification of Integrons Types and Extended-Spectrum Α-Lactamase Genes in Salmonella Species Collected From Pediatric Diarrheal Samples. Gazi Medical Journal, 32(3), 413-416.
Chicago	Eshaghi H, Alhooei S, Abbasi A, Khodabandeh M, Amiri FH, Pournajaf A, Kiani M, Gholami M, Hendizade P, Mohammadi M. "Pcr-Based Identification of Integrons Types and Extended-Spectrum Α-Lactamase Genes in Salmonella Species Collected From Pediatric Diarrheal Samples." Gazi Medical Journal 32, no. 3 (2021): 413-416.
Harvard	Eshaghi H et al. (2021) 'Pcr-Based Identification of Integrons Types and Extended-Spectrum Α-Lactamase Genes in Salmonella Species Collected From Pediatric Diarrheal Samples', Gazi Medical Journal, 32(3), pp. 413-416.
Vancouver	Eshaghi H, Alhooei S, Abbasi A, Khodabandeh M, Amiri FH, Pournajaf A, et al.. Pcr-Based Identification of Integrons Types and Extended-Spectrum Α-Lactamase Genes in Salmonella Species Collected From Pediatric Diarrheal Samples. Gazi Medical Journal. 2021;32(3):413-416.
BibTex	@article{ author = {Eshaghi H and Alhooei S and Abbasi A and Khodabandeh M and Amiri FH and Pournajaf A and Kiani M and Gholami M and Hendizade P and Mohammadi M}, title = {Pcr-Based Identification of Integrons Types and Extended-Spectrum Α-Lactamase Genes in Salmonella Species Collected From Pediatric Diarrheal Samples}, journal = {Gazi Medical Journal}, volume = {32}, number = {3}, pages = {413-416}, year = {2021} }
RIS	TY - JOUR AU - Eshaghi H AU - Alhooei S AU - Abbasi A AU - Khodabandeh M AU - Amiri FH AU - Pournajaf A AU - Kiani M AU - Gholami M AU - Hendizade P AU - Mohammadi M TI - Pcr-Based Identification of Integrons Types and Extended-Spectrum Α-Lactamase Genes in Salmonella Species Collected From Pediatric Diarrheal Samples JO - Gazi Medical Journal VL - 32 IS - 3 SP - 413 EP - 416 PY - 2021 ER -

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