Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Evaluation of the Effect of Mesenchymal Stem Cells Injection in the Nucleus Accumbens on the Morphine Reinstatement Behavior in a Conditioned Place Preference Model in Wistar Rat: Expression Changes of Nmda Receptor Subunits and Nt-3 Publisher Pubmed



Dousti Kataj P1 ; Vousooghi N2, 3, 4 ; Hadjighassem M5 ; Farahmandfar M1 ; Ebrahimibarough S6
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Neuroscience and Addiction Studies, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Research Center for Cognitive and Behavioral Sciences, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Iranian National Center for Addiction Studies, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Brain and Spinal Cord Injury Research Center, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Behavioural Brain Research Published:2023


Abstract

Mesenchymal stem cells (MSCs) have been recently shown to improve functional recovery in animal models of CNS disorders and are currently being examined in clinical studies for sclerosis, stroke, and CNS lesions. The activation of endogenous CNS protection and repair mechanisms is unclear. MSC-based approaches are considered a new potential target for neurodegenerative disorders. This study was designed to discover the effect of MSCs injection in the nucleus accumbens (NAc) on the reinstatement of behavior in morphine-induced conditioned place preference (CPP) in male rats. The CPP was induced via intra-peritoneal (i.p.) morphine injection (5 mg/kg) for three consecutive days. After being tested for CPP induction, animals received MSCs or culture medium (DMEM F-12) in their NAc using stereotaxic surgery. Following extinction, a priming dose of morphine (2 mg/kg) was administered to induce reinstatement. Expression of GluN1, GluN2A, and GluN2B subunits of the NMDA receptor and the NT-3 gene in the NAc was assessed on the last day of extinction and following CPP reinstatement. The results showed that local injection of MSCs attenuated reinstatement after receiving a priming dose of morphine, and also shortened the period of CPP extinction. The mRNA expression of the NT-3 gene in the group receiving MSCs was increased compared to control animals, as was observed for GluN1 and GluN2B, but not GluN2A. It is concluded that intra-NAc injection of MSCs may facilitate morphine extinction and alleviate reinstatement behavior which may be via expression changes in NMDA receptor subunits and NT-3 gene. © 2023 Elsevier B.V.
Related Docs
1. Nmda Receptor Subunits Change in the Prefrontal Cortex of Pure-Opioid and Multi-Drug Abusers: A Post-Mortem Study, European Archives of Psychiatry and Clinical Neuroscience (2019)
2. Expression of Nmda Receptor Subunits in Human Blood Lymphocytes: A Peripheral Biomarker in Online Computer Game Addiction, Journal of Behavioral Addictions (2018)
3. The Potential Role of the Orexin Reward System in Future Treatments for Opioid Drug Abuse, Brain Research (2020)
4. Preventing Morphine Reinforcement With High-Frequency Deep Brain Stimulation of the Lateral Hypothalamic Area, Addiction Biology (2019)
5. Opioid Reward and Deep Brain Stimulation of the Lateral Hypothalamic Area, Vitamins and Hormones (2025)
6. Exercise Can Restore Behavioural and Molecular Changes of Intergenerational Morphine Effects, Addiction Biology (2022)
7. Therapeutic Potential of Stem Cells for Treatment of Neurodegenerative Diseases, Biotechnology Letters (2020)
8. The Dorsal Hippocampal Group Iii Metabotropic Glutamate Receptors Are Involved in Morphine Effect on Memory Formation in Male Mice, European Journal of Pharmacology (2018)
Experts (# of related papers)
View all Related Experts
Esmail Riahi (3)
Nasim Vousooghi (2)