Spectroscopic and Molecular Docking Studies on Dna Binding Interaction of Podophyllotoxin

Spectroscopic and Molecular Docking Studies on Dna Binding Interaction of Podophyllotoxin

Heidary Alizadeh B¹ ; Dehghan GH² ; Derakhsh Ahmadi V³ ; Moghimi S⁴ ; Asadipour A⁵ ; Foroumadi A^{5, 6}

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1. Iranian Research Institute of Plant Protection (IRIPP), Tehran, Iran
2. Department of Biology, Faculty of Natural Science, University of Tabriz, Tabriz, Iran
3. Department of Pesticides, Iranian Research Institute of Plant Protection, Arak, Iran
4. Drug Design and Development Research Center, Tehran University of Medical Sciences, Tehran, Iran
5. Department of Medicinal Chemistry, Faculty of Pharmacy and Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, I-Kerman, Iran
6. Department of Medicinal Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran

Source: Journal of Sciences# Islamic Republic of Iran Published:2018

Abstract

The binding interaction of novel podophyllotoxin derivative, (3R, 4R)-4-((benzo[d][1, 3]dioxol-5-yl)methyl)-dihydro-3-(hydroxy(3, 4-dimethoxyphenyl) methyl) furan-2(3H)-one (PPT), with calf thymus DNA (ctDNA) has been examined using UV-Visible absorption spectrophotometry, fluorescence spectroscopy, viscosity measurement and molecular docking studies. UV-Vis absorption results showed hyperchromic effect and low binding constant value (1.01×104 M-1), indicating non-intercalative interaction as a binding mode. The competitive fluorescence study also confirmed the obtained results from UV-Vis absorption spectra. Small changes in the viscosity of DNA exhibited that the interaction of PPT with DNA is based on groove binding mode. Molecular docking study showed minor groove interaction and -7.08 kcal/mol as a calculated energy.

Style	Citing Format
MLA	Heidary Alizadeh B, et al.. "Spectroscopic and Molecular Docking Studies on Dna Binding Interaction of Podophyllotoxin." Journal of Sciences# Islamic Republic of Iran, vol. 29, no. 2, 2018, pp. 121-127.
APA	Heidary Alizadeh B, Dehghan GH, Derakhsh Ahmadi V, Moghimi S, Asadipour A, Foroumadi A (2018). Spectroscopic and Molecular Docking Studies on Dna Binding Interaction of Podophyllotoxin. Journal of Sciences# Islamic Republic of Iran, 29(2), 121-127.
Chicago	Heidary Alizadeh B, Dehghan GH, Derakhsh Ahmadi V, Moghimi S, Asadipour A, Foroumadi A. "Spectroscopic and Molecular Docking Studies on Dna Binding Interaction of Podophyllotoxin." Journal of Sciences# Islamic Republic of Iran 29, no. 2 (2018): 121-127.
Harvard	Heidary Alizadeh B et al. (2018) 'Spectroscopic and Molecular Docking Studies on Dna Binding Interaction of Podophyllotoxin', Journal of Sciences# Islamic Republic of Iran, 29(2), pp. 121-127.
Vancouver	Heidary Alizadeh B, Dehghan GH, Derakhsh Ahmadi V, Moghimi S, Asadipour A, Foroumadi A. Spectroscopic and Molecular Docking Studies on Dna Binding Interaction of Podophyllotoxin. Journal of Sciences# Islamic Republic of Iran. 2018;29(2):121-127.
BibTex	@article{ author = {Heidary Alizadeh B and Dehghan GH and Derakhsh Ahmadi V and Moghimi S and Asadipour A and Foroumadi A}, title = {Spectroscopic and Molecular Docking Studies on Dna Binding Interaction of Podophyllotoxin}, journal = {Journal of Sciences# Islamic Republic of Iran}, volume = {29}, number = {2}, pages = {121-127}, year = {2018} }
RIS	TY - JOUR AU - Heidary Alizadeh B AU - Dehghan GH AU - Derakhsh Ahmadi V AU - Moghimi S AU - Asadipour A AU - Foroumadi A TI - Spectroscopic and Molecular Docking Studies on Dna Binding Interaction of Podophyllotoxin JO - Journal of Sciences# Islamic Republic of Iran VL - 29 IS - 2 SP - 121 EP - 127 PY - 2018 ER -

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Abstract