A First Report of Hb Alesha [Β67(E11)Val>Met, Gtg>Atg] in an Iranian Patient Publisher Pubmed



Hamid M¹ ; Nezhad EZ² ; Galehdari H³ ; Saberi A³ ; Shariati G^{3, 4} ; Sedaghat A⁵
Source: Iranian Biomedical Journal Published:2019

Abstract

Background: Hemoglobin (Hb) Alesha is a rare and very unstable Hb variant, resulting in disruption of the heme pocket and producing severe hemolysis in heterozygous statues. In this study, we describe the first report of this variant in an Iranian boy originated from south of Iran with severe hemolytic anemia and mild splenomegaly. Methods: A six-year-old boy from Khuzestan Province and his parents were studied. Gap-PCR and direct sequencing were performed to detect the β-globin gene deletions and β-globin gene mutations, respectively. Results: The subject had a sporadic mutation GTG to ATG (Val [valine]>Met [methionine]) at codon 67 in heterozygous form on β-globin gene, which was not detected in his parents. Conclusion: Since both parents proved to be normal, this Hb variant could be considered as a de novo mutation, which is highly useful for prenatal diagnosis. © 2019, Pasteur Institute of Iran. All rights reserved.