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Ion-Pair Hollow Fiber Liquid-Phase Microextraction Combined With Capillary Electrophoresis for the Determination of Biogenic Amines in Rat Tissues Publisher Pubmed



Seyfinejad B1, 2 ; Jouyban K3 ; Dehpour AR3, 4 ; Mohammad Jafari R3 ; Charkhpour M5 ; Afshar Mogaddam MR6 ; Jouyban A2, 7
Authors
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Authors Affiliations
  1. 1. Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
  2. 2. Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
  3. 3. Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  6. 6. Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  7. 7. Faculty of Pharmacy, Near East University, North Cyprus, Mersin 10, Nicosia, 99138, Turkey

Source: Journal of Pharmaceutical and Biomedical Analysis Published:2022


Abstract

Herein, the development of an ion-pair hollow fiber liquid-phase microextraction (IP-HF-LPME) procedure followed by capillary electrophoresis (CE) with indirect UV detection for the simultaneous extraction and determination of aliphatic biogenic amines including spermidine, spermine, and cadaverine in tissue samples of rat is described. In this study, tissue samples were firstly homogenized with a cold trichloroacetic acid solution then the developed method was applied to the supernatant resulting from tissue homogenization. Different CE separation and indirect UV detection conditions, as well as IP-HF-LPME extraction conditions were studied in detail and optimized. A 11 mmol L−1 imidazole solution containing 13 % (v/v) ethanol adjusted at pH 3.3 (by acetic acid) was the best running buffer for indirect UV detection of non-UV-absorbing amines. 1-Octanol in combination with salicylic acid, respectively as the membrane solvent and ion-pair reagent provided the highest extraction recovery for the studied amines. Method performances were established and good results of linearity, recovery, sensitivity, and repeatability were achieved for all the studied amines. Limits of detection were found to be between 2.8 and 4.5 ng mL−1 and limits of quantification were 15 ng mL−1 and the dynamic range was 15–2000 ng mL−1 for all three analytes. © 2022 Elsevier B.V.