Molecular Characterization and Analysis of 16S Ribosomal Dna in Some Isolates of Demodex Folliculorum



Daneshparvar A¹ ; Mowlavi G¹ ; Mirjalali H² ; Hajjaran H¹ ; Mobedi I¹ ; Naddaf SR³ ; Shidfar M⁴ ; Sadat Makki M¹ Show Affiliations
Source: Iranian Journal of Parasitology Published:2017

Abstract

Background: Demodicosis is one of the most prevalent skin diseases resulting from infestation by Demodex mites. This parasite usually inhabits in follicular infun-dibulum or sebaceous duct transmitted through close contact with an infested host. Methods: This study was carried from September 2014 to January 2016 at Tehran University of Medical Sciences, Tehran, Iran. DNA extraction and amplification of 16S ribosomal RNA was performed on four isolates, obtained from four patients and identified morphologically through clearing with 10% Potassium hydroxide (KOH) and microscopical examination. Amplified fragments from the isolates were compared with GenBank database and phylogenetic analysis was carried out using MEGA6 software. Results: A 390 bp fragment of 16S rDNA was obtained in all isolates and analysis of generated sequences showed high similarity with those submitted to GenBank, previously. Intra-species similarity and distance also showed 99.983% and 0.017, respectively, for the studied isolates. Multiple alignments of the isolates showed Single Nucleotide Polymorphisms (SNPs) in 16S rRNA fragment. Phylogenetic analysis revealed that all 4 isolates clustered with other D. folliculorum, recovered from GenBank database. Our accession numbers KF875587 and KF875589 showed more similarity together in comparison with two other studied isolates. Conclusion: Mitochondrial 16S rDNA is one of the most suitable molecular bar-codes for identification D. folliculorum and this fragment can use for intra-species characterization of the most human-infected mites. © 2017, Tehran University of Medical Sciences (TUMS). All rights reserved.