Chitosan Glutamate Nanoparticles Loaded With Nisin for Antibacterial and Anticancer Studies Publisher



S ; Komijani S ; Sharifian G ; Hosseini F ; Mohajer F
Source: International Journal of Biological Macromolecules Published:2025

Abstract

Cancer is a major cause of death, and current treatments face challenges like resistance and side effects. Nisin, an antimicrobial peptide, shows promise as an anti-cancer agent but needs better delivery methods. This study investigates chitosan-glutamate nanoparticles as a carrier for nisin. The nanoparticles, synthesized by ionic gelation, were characterized by various techniques (DLS, XRD, FT-IR, EDX, SEM). The nisin-loaded nanoparticles were 148.8 nm in size, non-spherical, and porous. Nisin encapsulation was 92 %, and they showed superior antibacterial activity against S. aureus and E. coli. In cancer cell tests, these nanoparticles reduced the viability of 4 T1 breast cancer cells to 29.58 % without harming normal cells. Nisin's IC50 was halved to 2.5 mg/ml after encapsulation. These results suggest that chitosan-glutamate nanoparticles are an effective carrier for nisin, offering both antibacterial and anti-cancer potential. © 2025 Elsevier B.V., All rights reserved.