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Lymphoid Lineage Differentiation Potential of Mouse Nuclear Transfer Embryonic Stem Cells Publisher Pubmed



Eslamiarshaghi T1 ; Salehi M2, 3 ; Soleimani M4 ; Gholipourmalekabadi M3 ; Mossahebimohammadi M4, 5 ; Ardeshirylajimi A6 ; Rajabi H1
Authors
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Authors Affiliations
  1. 1. Department of Transgenic Animal Sciences, Stem Cells Technology Research Center, Tehran, Iran
  2. 2. Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  3. 3. Biotechnology Department, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  4. 4. Department of Hematology and Blood Banking, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  5. 5. Urogenital Stem Cell Research Center (UGSCRC), Shahid Beheshti University of Medical Sciences, Tehran, Iran
  6. 6. Department of Stem Cell Biology, Stem Cells Technology Research Center, Tehran, Iran

Source: Biologicals Published:2015


Abstract

Stem cells therapy is considered as an efficient strategy for the treatment of some diseases. Nevertheless, some obstacles such as probability of rejection by the immune system limit applications of this strategy. Therefore, several efforts have been made to overcome this among which using the induced pluripotent stem cells (iPSCs) and nuclear transfer embryonic stem cell (nt-ESCs) are the most efficient strategies. The objective of this study was to evaluate the differentiation potential of the nt-ESCs to lymphoid lineage in the presence of IL-7, IL-3, FLT3-ligand and TPO growth factors in vitro. To this end, the nt-ESCs cells were prepared and treated with aforementioned growth factors for 7 and 14 days. Then, the cells were examined for expression of lymphoid markers (CD3, CD25, CD127 and CD19) by quantitative PCR (q-PCR) and flow cytometry. An increased expression of CD19 and CD25 markers was observed in the treated cells compared with the negative control samples by day 7. After 14 days, the expression level of all the tested CD markers significantly increased in the treated groups in comparison with the control. The current study reveals the potential of the nt-ESCs in differentiation to lymphoid lineage in the presence of defined growth factors. © 2015 The International Alliance for Biological Standardization.
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