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Maldi-Tof Ms Characterisation, Genetic Diversity and Antifungal Susceptibility of Trichosporon Species From Iranian Clinical Samples Publisher Pubmed



Ahangarkani F1, 2 ; Ilkit M3 ; Vaseghi N4 ; Zahedi N2 ; Zomorodian K5 ; Khodavaisy S6 ; Afsarian MH7 ; Abbasi K8 ; De Groot T1 ; Meis JF1, 9, 10 ; Badali H2, 11, 12
Authors
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Authors Affiliations
  1. 1. Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Nijmegen, Netherlands
  2. 2. Antimicrobial Resistance Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran
  3. 3. Division of Mycology, Department of Microbiology, Faculty of Medicine, University of Cukurova, Adana, Turkey
  4. 4. Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
  5. 5. Basic Sciences in Infectious Diseases Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
  6. 6. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Department of Medical Mycology and Parasitology, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran
  8. 8. Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran
  9. 9. ECMM Excellence Center for Medical Mycology, Centre of Expertise in Mycology Radboudumc/ Canisius-Wilhelmina Hospital, Nijmegen, Netherlands
  10. 10. Bioprocess Engineering and Biotechnology Graduate Program, Federal University of Parana, Curitiba, Brazil
  11. 11. Department of Medical Mycology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  12. 12. Fungus Testing Laboratory, Department of Pathology and Laboratory Medicine, University of Texas Health Science Center at San Antonio, San Antonio, TX, United States

Source: Mycoses Published:2021


Abstract

Background: Trichosporonosis is an emerging fungal infection caused by Trichosporon species, a genus of yeast-like fungi, which are frequently encountered in human infections ranging from mild cutaneous lesions to fungemia in immunocompromised patients. The incidence of trichosporonosis has increased in recent years, owing to higher numbers of individuals at risk for this infection. Although amphotericin B, posaconazole and isavuconazole are generally effective against Trichosporon species, some isolates may have variable susceptibility to these antifungals. Objectives: Herein, we evaluated the species distribution, genetic diversity and antifungal susceptibility profiles of Trichosporon isolates in Iran. Methods: The yeasts were identified by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS). Phylogenetic analysis was performed based on amplified fragment length polymorphism (AFLP). The in vitro susceptibilities of eight antifungal agents were analysed using the Clinical and Laboratory Standards Institute broth microdilution methods. Results: The isolates belonged to the species T asahii (n = 20), T japonicum (n = 4) and T faecale (n = 3). A dendrogram of the AFLP analysis demonstrated that T asahii and non-asahii Trichosporon strains (T japonicum and T faecale) are phylogenetically distinct. While voriconazole was the most active agent (GM MIC = 0.075 μg/ml), high fluconazole MICs (8 μg/ml) were observed for a quarter of Trichosporon isolates. The GM MIC value of amphotericin B for T asahii and non-asahii Trichosporon species was 0.9 μg/ml. Conclusions: The distribution and antifungal susceptibility patterns of the identified Trichosporon species could inform therapeutic choices for treating these emerging life-threatening fungi. © 2021 Wiley-VCH GmbH
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