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Network Analysis of Effect of Light-Dark Time Ratio on the Gene Expression Profile of Mouse Skin Publisher



Arjmand B1 ; Hamzeloomoghadam M2 ; Razzaghi Z3 ; Nejad MR4 ; Azodi MZ5 ; Khodadoost M2 ; Tavirani MR6 ; Tavirani MR6 ; Ahmadzadeh A8
Authors
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Authors Affiliations
  1. 1. Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Traditional Medicine and Materia Medica Research Center, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  3. 3. Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  4. 4. Research Institute for Gastroenterology and Liver Diseases, Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  5. 5. Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  6. 6. Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  7. 7. Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
  8. 8. Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Journal of Lasers in Medical Sciences Published:2022


Abstract

Introduction: Circadian rhythms refer to daily cyclic events such as activity and rest in biology. A protein-based core related to the mechanism of circadian is identified. In the present study, the gene expression profiles of mouse skin in different conditions of light-dark times were investigated via protein-protein interaction (PPI) analysis to explore the main affected genes. Methods: GSE174155 was derived from Gene Expression Omnibus (GEO) and was analyzed via GEO2R to find the significant differentially expressed genes (DEGs). The gene expression profiles of Cry-null (genotype: cryptochrome-1(-/-): crytochrome-2 (-/-)) mouse skin versus the wild-type samples in the various circadian times (CTs) were assessed. The queried DEGs plus 50 first neighbors were included in a PPI network via the STRING database by Cytoscape software. The networks were analyzed and the central nodes were evaluated. Results: Three groups of mice based on CTs were identified. 15, 15, and 14 central nodes were determined as central nodes for the analyze networks. There was not a common central node for the analyzed networks. Conclusion: It was pointed out that the light/dark time ratio had a gross effect on the gene expression profile of the skin in the mice. Results imply more investigations to suggest a standard protocol related to CT, considering human lifestyle and exploring suitable protective methods for the jobs which are fixed in the abnormal CT sets. © 2022,Journal of Lasers in Medical Sciences. All Rights Reserved.