Ribosomal Dna Internal Transcribed Spacer 2 Sequence Analysis and Phylogenetic Comparison of Seven Cockroach Species in Northwestern Iran

Ribosomal Dna Internal Transcribed Spacer 2 Sequence Analysis and Phylogenetic Comparison of Seven Cockroach Species in Northwestern Iran Publisher Pubmed

Farmani M^{1, 2} ; Basseri H³ ; Norouzi B⁴ ; Gholizadeh S^{1, 2}

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1. Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran
2. Medical Entomology Department, School of Public Health, Urmia University of Medical Sciences, Urmia, Iran
3. Medical Entomology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
4. Research Center of Health and Environment, Guilan University of Medical Sciences, Rasht, Iran

Source: BMC Research Notes Published:2019

Abstract

Objectives: The current study was conducted to identify cockroach species (Blattodea) of northwestern Iran in public places using morphological characteristics and ribosomal DNA internal transcribed spacer 2 (rDNA-ITS2). Sequences were analyzed with Basic Local Alignment Search Tool (BLAST) searches, Neighbor-Joining methods based on and Tamura-Nei phylogenetic analyses. In addition, eight cockroach rDNA-ITS2 sequences from China, India, Iran and the United States obtained from GenBank were compared to those obtained in this study. Results: Specimens collected in Iran were identified as Periplaneta americana (L.), Shelfordella lateralis (Walker), Blatta orientalis (L.) (Blattodea: Blattidae), Blattella germanica (L.), Supella longipalpa (F.) (Blattodea: Ectobiidae), Polyphaga aegyptiaca (L.), and Polyphaga saussurei (Dohrn) (Blattodea: Corydiidae). rDNA-ITS2 nucleotide sequence analysis showed 100% similarity between P. aegyptiaca and P. saussurei species collected from Iran despite morphological differences. However, ITS2 sequence of P. americana submitted from China showed 30.49-31.71% difference to P. americana sequences from Iran and the United States. The results highlight the importance of morphological identification of cockroach species before conducting molecular techniques. © 2019 The Author(s).

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Mohammad Ali Oshaghi (2)

Kamran Akbarzadeh (1)

Style	Citing Format
MLA	Farmani M, et al.. "Ribosomal Dna Internal Transcribed Spacer 2 Sequence Analysis and Phylogenetic Comparison of Seven Cockroach Species in Northwestern Iran." BMC Research Notes, vol. 12, no. 1, 2019, pp. -.
APA	Farmani M, Basseri H, Norouzi B, Gholizadeh S (2019). Ribosomal Dna Internal Transcribed Spacer 2 Sequence Analysis and Phylogenetic Comparison of Seven Cockroach Species in Northwestern Iran. BMC Research Notes, 12(1), -.
Chicago	Farmani M, Basseri H, Norouzi B, Gholizadeh S. "Ribosomal Dna Internal Transcribed Spacer 2 Sequence Analysis and Phylogenetic Comparison of Seven Cockroach Species in Northwestern Iran." BMC Research Notes 12, no. 1 (2019): -.
Harvard	Farmani M et al. (2019) 'Ribosomal Dna Internal Transcribed Spacer 2 Sequence Analysis and Phylogenetic Comparison of Seven Cockroach Species in Northwestern Iran', BMC Research Notes, 12(1), pp. -.
Vancouver	Farmani M, Basseri H, Norouzi B, Gholizadeh S. Ribosomal Dna Internal Transcribed Spacer 2 Sequence Analysis and Phylogenetic Comparison of Seven Cockroach Species in Northwestern Iran. BMC Research Notes. 2019;12(1):-.
BibTex	@article{ author = {Farmani M and Basseri H and Norouzi B and Gholizadeh S}, title = {Ribosomal Dna Internal Transcribed Spacer 2 Sequence Analysis and Phylogenetic Comparison of Seven Cockroach Species in Northwestern Iran}, journal = {BMC Research Notes}, volume = {12}, number = {1}, pages = {-}, year = {2019} }
RIS	TY - JOUR AU - Farmani M AU - Basseri H AU - Norouzi B AU - Gholizadeh S TI - Ribosomal Dna Internal Transcribed Spacer 2 Sequence Analysis and Phylogenetic Comparison of Seven Cockroach Species in Northwestern Iran JO - BMC Research Notes VL - 12 IS - 1 SP - EP - PY - 2019 ER -

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