The Modulatory Effects of Vitamin D on the Expression of Il-12 and Tgf-Β in the Spinal Cord and Serum of Mice With Experimental Autoimmune Encephalomyelitis

The Modulatory Effects of Vitamin D on the Expression of Il-12 and Tgf-Β in the Spinal Cord and Serum of Mice With Experimental Autoimmune Encephalomyelitis

Ahangarparvin R^{1, 2} ; Mohammadikordkhayli M³ ; Azizi SV^{1, 2} ; Nemati M^{4, 5} ; Khorramdelazad H^{1, 2} ; Taghipour Z⁶ ; Hassan Z⁷ ; Moazzeni SM⁷ ; Jafarzadeh A^{1, 2}

Show Affiliations

1. Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
2. Dept. of Immunology, Medical School, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
3. Dept. of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
4. Dept. of Laboratory Sciences, Para-Medical School, Kerman University of Medical Sciences, Kerman, Iran
5. Dept. of Immunology, Medical School, Kerman University of Medical Sciences, Kerman, Iran
6. Dept. of Histology, Medical School, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
7. Dept. of Immunology, Medical School, Tarbiat Moddares University, Tehran, Iran

Source: Iranian Journal of Pathology Published:2018

Abstract

Background & objective: The immunoregulatory effects of transforming growth factor (TGF)-βand interleukin-12 (IL-12) and immunomodulatory actions of vitamin D (VD) were reported in several studies. This study aims to evaluate VD effects on IL-12 and TGF-β expression in experimental autoimmune encephalomyelitis (EAE). Methods: EAE was induced in three groups of C57BL/6 mice by immunization with MOG and administered intra-peritoneally 200 ngVD, PBS or olive oil (OO) from day +3 to +30. One group was also considered as healthy control group. At day 31, cytokines expression in the spinal cord and their serum levels were determined using real time-PCR and ELISA, respectively. Results: IL-12 gene expression and its serum levels in PBS-injected- or OO-administrated EAE groups were significantly higher than healthy group. IL-12 gene expression in EAE group treated with VD was significantly decreased compared to PBS-injected- or OO-administrated EAE groups (P<0.001 and P<0.02, respectively). Serum IL-12 levels were significantly reduced in VD-treated EAE mice compared to PBS-injected- or OO-administrated EAE groups (P<0.004 and P<0.05, respectively). No significant difference was observed between PBS-injected-EAE, OO-administrated EAE and healthy groups regarding the expression of TGF-β. In EAE mice treated with VD, TGF-β expression was significantly higher than healthy group and PBS-injected EAE mice (P<0.01 and P<0.04, respectively). VD-treated EAE group had also higher serum TGF-β levels than PBS-injected-, OO-administrated and healthy groups (P<0.05). Conclusion: VD modulates the expression of IL-12 and TGF-β in spinal cord and serum of EAE mice. © 2017, IRANIAN JOURNAL OF PATHOLOGY.

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Style	Citing Format
MLA	Ahangarparvin R, et al.. "The Modulatory Effects of Vitamin D on the Expression of Il-12 and Tgf-Β in the Spinal Cord and Serum of Mice With Experimental Autoimmune Encephalomyelitis." Iranian Journal of Pathology, vol. 13, no. 1, 2018, pp. 10-22.
APA	Ahangarparvin R, Mohammadikordkhayli M, Azizi SV, Nemati M, Khorramdelazad H, Taghipour Z, Hassan Z, Moazzeni SM, Jafarzadeh A (2018). The Modulatory Effects of Vitamin D on the Expression of Il-12 and Tgf-Β in the Spinal Cord and Serum of Mice With Experimental Autoimmune Encephalomyelitis. Iranian Journal of Pathology, 13(1), 10-22.
Chicago	Ahangarparvin R, Mohammadikordkhayli M, Azizi SV, Nemati M, Khorramdelazad H, Taghipour Z, Hassan Z, Moazzeni SM, Jafarzadeh A. "The Modulatory Effects of Vitamin D on the Expression of Il-12 and Tgf-Β in the Spinal Cord and Serum of Mice With Experimental Autoimmune Encephalomyelitis." Iranian Journal of Pathology 13, no. 1 (2018): 10-22.
Harvard	Ahangarparvin R et al. (2018) 'The Modulatory Effects of Vitamin D on the Expression of Il-12 and Tgf-Β in the Spinal Cord and Serum of Mice With Experimental Autoimmune Encephalomyelitis', Iranian Journal of Pathology, 13(1), pp. 10-22.
Vancouver	Ahangarparvin R, Mohammadikordkhayli M, Azizi SV, Nemati M, Khorramdelazad H, Taghipour Z, et al.. The Modulatory Effects of Vitamin D on the Expression of Il-12 and Tgf-Β in the Spinal Cord and Serum of Mice With Experimental Autoimmune Encephalomyelitis. Iranian Journal of Pathology. 2018;13(1):10-22.
BibTex	@article{ author = {Ahangarparvin R and Mohammadikordkhayli M and Azizi SV and Nemati M and Khorramdelazad H and Taghipour Z and Hassan Z and Moazzeni SM and Jafarzadeh A}, title = {The Modulatory Effects of Vitamin D on the Expression of Il-12 and Tgf-Β in the Spinal Cord and Serum of Mice With Experimental Autoimmune Encephalomyelitis}, journal = {Iranian Journal of Pathology}, volume = {13}, number = {1}, pages = {10-22}, year = {2018} }
RIS	TY - JOUR AU - Ahangarparvin R AU - Mohammadikordkhayli M AU - Azizi SV AU - Nemati M AU - Khorramdelazad H AU - Taghipour Z AU - Hassan Z AU - Moazzeni SM AU - Jafarzadeh A TI - The Modulatory Effects of Vitamin D on the Expression of Il-12 and Tgf-Β in the Spinal Cord and Serum of Mice With Experimental Autoimmune Encephalomyelitis JO - Iranian Journal of Pathology VL - 13 IS - 1 SP - 10 EP - 22 PY - 2018 ER -

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