Molecular Investigation of Campylobacter Spp. and Salmonella Infection in Children With Community Acquired Diarrhea

Molecular Investigation of Campylobacter Spp. and Salmonella Infection in Children With Community Acquired Diarrhea

Ahadi H¹ ; Attaran B¹ ; Ghanaie RM² ; Ganji L³ ; Fallah F² ; Karimi A² ; Sedighi I⁴ ; Tariverdi M⁵ ; Nateghian A⁶ ; Moghadam N⁷ ; Alebouyeh M²

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1. Dept. of microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
2. Division of Microbiology, Dept. of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
3. Pediatric Infection Research Center, Research Institue for Childrenâ€™s Health, Shahid Beheshti University of Medical Science, Tehran, Iran
4. Dept. of Pediatrics, School of Medicine, Hamadan University of Medical Sciences, Iran
5. Dept. of Pediatrics, Clinical Research Center of Hormozgan University of Medical Sciences, Bandar Abbas, Iran
6. Dept. of Pediatrics, Hazrat Ali Asghar Hospital, Tehran, Iran
7. Dept. of Pediatrics, School of Medicine, Bouali Hospital, Ardabil University of Medical Sciences, Iran

Source: Koomesh Published:2022

Abstract

Introduction: Acute gastroenteritis is a typical disorder that accounts for 8-12% of pediatric outpatient visits. Campylobacter and Salmonella infections account for about 8.4% and 11% of global diarrhea cases. Due to the importance of these bacteria in pediatric diseases, the aim of this study was to determine the infectious rate of Salmonella and Campylobacter species and also the frequency of the gene encoding Cytholethal distending toxin in children with community-acquired diarrhea. Materials and Methods: Stool samples of children under 5 years of age with diarrhea were collected. The samples were related to children referred to hospitals in Hamadan, Ardabil, Bandar Abbas and two hospitals in Tehran. DNA was extracted from the samples using a DNA extraction kit from stool. The presence of Campylobacter in the studied samples was detected by polymerase chain reaction using specific primers. A control stool sample was spiked with 10-fold dilution of C. jejuni suspension for LOD (detection limit determination) measurement. Results: In this study, PCR results showed a LOD of 100 CFU per gram in the spiked feces sample. Accordingly, out of 144 fecal samples of children with acute diarrhea, one case was positive for Campylobacter jejuni; this sample was also positive for the presence of cdtB gene. Presence of Salmonella was confirmed in two samples of the patients (1.4%). Conclusion: Low prevalence of Campylobacter and Salmonella was detected in symptomatic children under 5 years of age during the Covid-19 pandemic. Examination of these samples for viruses and other microbial agents can clarify the etiology of diarrhea in children referred to the hospitals. © 2022, Semnan University of Medical Sciences. All rights reserved.

Style	Citing Format
MLA	Ahadi H, et al.. "Molecular Investigation of Campylobacter Spp. and Salmonella Infection in Children With Community Acquired Diarrhea." Koomesh, vol. 24, no. 5, 2022, pp. 684-690.
APA	Ahadi H, Attaran B, Ghanaie RM, Ganji L, Fallah F, Karimi A, Sedighi I, Tariverdi M, Nateghian A, Moghadam N, Alebouyeh M (2022). Molecular Investigation of Campylobacter Spp. and Salmonella Infection in Children With Community Acquired Diarrhea. Koomesh, 24(5), 684-690.
Chicago	Ahadi H, Attaran B, Ghanaie RM, Ganji L, Fallah F, Karimi A, Sedighi I, et al.. "Molecular Investigation of Campylobacter Spp. and Salmonella Infection in Children With Community Acquired Diarrhea." Koomesh 24, no. 5 (2022): 684-690.
Harvard	Ahadi H et al. (2022) 'Molecular Investigation of Campylobacter Spp. and Salmonella Infection in Children With Community Acquired Diarrhea', Koomesh, 24(5), pp. 684-690.
Vancouver	Ahadi H, Attaran B, Ghanaie RM, Ganji L, Fallah F, Karimi A, et al.. Molecular Investigation of Campylobacter Spp. and Salmonella Infection in Children With Community Acquired Diarrhea. Koomesh. 2022;24(5):684-690.
BibTex	@article{ author = {Ahadi H and Attaran B and Ghanaie RM and Ganji L and Fallah F and Karimi A and Sedighi I and Tariverdi M and Nateghian A and Moghadam N and Alebouyeh M}, title = {Molecular Investigation of Campylobacter Spp. and Salmonella Infection in Children With Community Acquired Diarrhea}, journal = {Koomesh}, volume = {24}, number = {5}, pages = {684-690}, year = {2022} }
RIS	TY - JOUR AU - Ahadi H AU - Attaran B AU - Ghanaie RM AU - Ganji L AU - Fallah F AU - Karimi A AU - Sedighi I AU - Tariverdi M AU - Nateghian A AU - Moghadam N AU - Alebouyeh M TI - Molecular Investigation of Campylobacter Spp. and Salmonella Infection in Children With Community Acquired Diarrhea JO - Koomesh VL - 24 IS - 5 SP - 684 EP - 690 PY - 2022 ER -

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