Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Genotyping and Molecular Analysis of Enterocytozoon Bieneusi Isolated From Immunocompromised Patients in Iran Publisher Pubmed



Mirjalali H1, 2 ; Mirhendi H3 ; Meamar AR4 ; Mohebali M1, 2 ; Askari Z1 ; Mirsamadi ES5 ; Rezaeian M1, 2
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Medical Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Infection, Genetics and Evolution Published:2015


Abstract

Microsporidia are known as opportunistic unicellular pathogens, particularly so in individuals with congenital or acquired immunodeficiency. Enterocytozoon bieneusi is one of the most common species infecting both immunocompromised and immunocompetent individuals. The aim of this study was to assess the distribution of E. bieneusi genotypes among immunocompromised patients in Iran. From 329 stool samples referred for parasitological analysis during 2011-2014, 14 samples from immunocompromised patients proving positive for E. bieneusi by SSU rDNA analysis were selected. Genotyping was carried out using specific primers targeting the Internal Transcribed Spacer (ITS) region. Subsequently, all samples were sequenced and results queried against the GenBank database. Moreover, sequences were subject to phylogenetic analysis. The expected amplification product was generated for all samples. Genotype D was identified in patients with HIV +/AIDS, transplant recipients, and cancer patients, while Genotype E was identified only in cancer and HIV +/AIDS patients. Phylogenetic analysis revealed that there was no relationship between genotypes and types of immunosuppression, whereas most genotype D isolates grouped with those described previously from cattle, horses, birds, and humans. E. bieneusi genotype D appears to be the most frequent genotype in immunocompromised patients, while Genotype E was observed only in HIV +/AIDS patients and cancer patients, not transplant recipients. © 2015.
Related Docs
1. Intestinal Parasites Associated With Opportunistic Coccidial Infections Among Immunocompromised Individuals in Central Iran: A Cross Sectional Study, Archives of Clinical Infectious Diseases (2019)
2. Prevalence, Molecular Characteristics and Risk Factors for Cryptosporidiosis Among Iranian Immunocompromised Patients, Microbiology and Immunology (2012)
3. Genetic Mutations and Immunological Features of Severe Combined Immunodeficiency Patients in Iran, Immunology Letters (2019)
4. Prevalence of Intestinal Parasitic Infection Among Inhabitants and Tribes of Chelgerd, Iran, 2008-2009, Journal of Clinical and Diagnostic Research (2015)
5. Frequency and Molecular Identification of Cryptosporidium Species Among Immunocompromised Patients Referred to Hospitals, Central Iran, 2015-16, Iranian Journal of Parasitology (2020)
6. Genomic Testing Identifies Monogenic Causes in Patients With Very Early-Onset Inflammatory Bowel Disease: A Multicenter Survey in an Iranian Cohort, Clinical and Experimental Immunology (2024)
Experts (# of related papers)
View all Related Experts
Zahra Ghayour Najafabadi (2)
Tooba Momen (2)