Isfahan University of Medical Sciences

Science Communicator Platform

Share By
Investigating the in Vivo Effects of the Human Adipose Tissue-Derived Stem Cell Transplantation and Bromelain in Myelin Regeneration Publisher

Summary: Could bromelain aid myelin repair? In an experimental MS model, bromelain with stem cells improved myelination and balance; effects in humans remain unknown. #MultipleSclerosis #StemCells

Rahnama S ; Ghasemi N
Authors

Source: Research in Pharmaceutical Sciences Published:2026


Abstract

Background and purpose: Natural compounds combined with human adipose-derived stem cell (hADSCs) transplantation could be considered an effective treatment for multiple sclerosis (MS). In the present study, the effects of bromelain administration in combination with hADSCs transplantation were evaluated for oligodendrocyte differentiation, myelin tissue regeneration, and improvement of motor function. Experimental approach: After inducing an MS mouse model, the mice were randomly divided into 4 groups: MS, MS + bromelain, MS + stem cell, and MS + bromelain + stem cell. Besides, 16 mice were designated for the control and sham groups. Finally, the behavioral test is used to evaluate motor function and motor defects. Furthermore, immunofluorescent staining was used to evaluate the cell markers. The serum levels of IgG, as well as inflammatory and anti-inflammatory factors, were assessed using an ELISA test. Findings/Results: The mean percentages of Olig2- and Mog-positive cells, myelin density, and the serum levels of IL-10 and TGF-β were significantly higher in the treated groups than in the MS group. However, the serum levels of IL-17 and IFN-γ in the MS group were significantly higher compared to the other groups. Also, the serum level of IgG in the MS group was significantly higher than in the other groups. Conclusion and implications: It can be said that hADSCs transplantation, along with bromelain administration, is able to increase myelination and improve balance function. Therefore, the use of these agents could be a fundamental step towards finding a suitable method for the treatment of neurodegenerative diseases. © 2026 Research in Pharmaceutical Sciences.
Related Docs
View other Related Docs
1. Synergistic Myelin Regenerative, Immunomodulatory and Anti-Inflammatory Effects of Vanillin and Adipose Derived Stem Cells in Cuprizone Animal Model of Multiple Sclerosis, Iranian Journal of Basic Medical Sciences (2026)
2. The Immunoregulatory and Neuroprotective Effects of Human Adipose Derived Stem Cells Overexpressing Il-11 and Il-13 in the Experimental Autoimmune Encephalomyelitis Mice, International Immunopharmacology (2020)
3. Evaluation of Edaravone Effects on the Differentiation of Human Adipose Derived Stem Cells Into Oligodendrocyte Cells in Multiple Sclerosis Disease in Rats, Life Sciences (2021)
Experts (# of related papers)
View all Related Experts
Nazem Ghasemi (9)
Mohammad Mardani (6)
Other Related Docs
4. Therapeutic Effects of Extracellular Vesicles From Human Adipose-Derived Mesenchymal Stem Cells on Chronic Experimental Autoimmune Encephalomyelitis, Journal of Cellular Physiology (2020)
5. Transplantation of Human Adipose-Derived Stem Cells Overexpressing Lif/Ifn-Β Promotes Recovery in Experimental Autoimmune Encephalomyelitis (Eae), Scientific Reports (2022)
6. Evaluation of in Vivo Lithium Chloride Effects As a Gsk3-Β Inhibitor on Human Adipose Derived Stem Cells Differentiation Into Oligodendrocytes and Re-Myelination in an Animal Model of Multiple Sclerosis, Molecular Biology Reports (2023)
7. Transplantation of Human Adipose-Derived Stem Cells Enhances Remyelination in Lysolecithin-Induced Focal Demyelination of Rat Spinal Cord, Molecular Biotechnology (2014)
8. Evaluating the Effect of Il-27-Transfected Mesenchymal Stem Cells on Certain Histological and Immunological Parameters in a Mouse Model of Experimental Autoimmune Encephalomyelitis, Journal of Isfahan Medical School (2013)
9. Improvement of Remyelination in Demyelinated Corpus Callosum Using Human Adipose-Derived Stem Cells (Hadscs) and Pregnenolone in the Cuprizone Rat Model of Multiple Sclerosis, Journal of Molecular Neuroscience (2020)
10. Impact of Intraventricular Human Adipose-Derived Stem Cells Transplantation With Pregnenolone Treatment on Remyelination of Corpus Callosum in a Rat Model of Multiple Sclerosis, Cell Journal (2022)
11. Significant Immunomodulatory and Hepatoprotective Impacts of Silymarin in Ms Patients: A Double-Blind Placebo-Controlled Clinical Trial, International Immunopharmacology (2021)
12. Valproic Acid Effects on Human Adipose-Derived Stem Cell Differentiation Into Oligodendrocytes and Improved Remyelination in a Mouse Model of Multiple Sclerosis, International Journal of Developmental Biology (2023)
13. Co-Transplantation of Human Neurotrophic Factor Secreting Cells and Adipose-Derived Stem Cells in Rat Model of Multiple Sclerosis, Cell Journal (2018)
14. Dimethyl Fumarate As an Immunomodulatory Enhancer of Adipose-Derived Mesenchymal Stem Cells: Therapeutic Implications, Cell and Tissue Banking (2026)
15. The Evaluation of Astaxanthin Effects on Differentiation of Human Adipose Derived Stem Cells Into Oligodendrocyte Precursor Cells, Avicenna Journal of Medical Biotechnology (2018)
16. The Role of Cobalamin on Interleukin 10, Osteopontin, and Related Micrornas in Multiple Sclerosis, Iranian Journal of Allergy, Asthma and Immunology (2022)