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A Simple and Low-Cost Method for Determination of Methanol in Alcoholic Solutions Publisher



Khodadadi M1 ; Badibostan H2 ; Neisiani AK2 ; Sabzghabaee AM2 ; Meamar R2 ; Giesy JP3, 4, 5 ; Eizadimood N2
Authors
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Authors Affiliations
  1. 1. Core Research Facilities (CRF), Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Isfahan Clinical Toxicology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Veterinary Biomedical Sciences and Toxicology Centre, University of Saskatchewan, Saskatoon, S7N 5B3, SK, Canada
  4. 4. Department of Integrative Biology and Center for Integrative Toxicology, Michigan State University, 1129 Farm Lane Road, East Lansing, MI, United States
  5. 5. Department of Environmental Sciences, Baylor University, Waco, 76706, TX, United States

Source: Toxicology Reports Published:2024


Abstract

Methanol poisoning can occur through consumption of methanol-containing alcohols, especially in areas where production, distribution, sale and consumption of alcohol is lawfully prohibited. Due to its toxic potency, determination of methanol in alcoholic solutions is important. The aim of the present study was to develop a rapid, simple and inexpensive method for quantification of methanol in alcoholic solutions that uses minimal equipment available in most laboratories. The method developed is based microdistillation and chromotropic acid, which can be conducted without sophisticated instruments or personal. The system consists of a micro-tube suspended in a falcon tube to function as a collector. Methanol is separated from wine by microdistillation at 90°C in water bath and converted to formaldehyde in the collector. The collector contains an acidic permanganate solution that converts methanol to formaldehyde. Formaldehyde was then quantified by use of chromotropic acid in concentrated sulfuric acid. Experimental variables were optimized by using central composite design (CCD). Method detection and quantification limits were 183 mg L−1 and 584 mg L−1, respectively. The percent relative standard deviation (RSD%) were between 6.4 and 7.9. Accuracies were between 89.6 % and 92.4 %. Concentrations of methanol in five alcoholic solutions were between 2.9×104 and 3.0×104, mg/L, v/v (ppm). Due to its simplicity and cost effectiveness, this method can be used for routine, real-time determination of methanol in alcoholic solutions. © 2024 The Authors