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A Simple, Efficient and Inexpensive Method for Malaria Parasites’ Dna Catching From Fixed Giemsa-Stained Blood Slides Publisher



Eskandarian AA1 ; Moradi S2 ; Abedi S2
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Authors Affiliations
  1. 1. Department of Parasitology and Mycology, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Departments of Microbiology, Faculty of Biological Sciences, Islamic Azad University, Falavarjan Branch, Isfahan, Iran

Source: Journal of Parasitic Diseases Published:2016


Abstract

As parasitological or microscopic method is the gold standard and the best method for diagnosis of malaria, so fixed Geimsa-stained blood slides in the form of thick and thin blood smears are the most important data collections of malaria, especially historical slides. The parasites are dead but their DNA is valuable for many molecular biologic researches. A simple and efficient method for catching and extraction malaria parasites' DNA with a desired yield from dried and stained blood on slides is the first and major step. Introduction of an applicable, efficient and inexpensive DNA catching method and assessment of its performance in following molecular applications was the main objective of present study. © 2014, Indian Society for Parasitology.
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Abbas Ali Eskandarian (2)
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