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Prevalence and Risk Factor Analysis of Haemobartonella Felis in Cats Using Direct Blood Smear and Pcr Assay Publisher



Torkan S1 ; Aldavood SJ2 ; Rafie SM1 ; Hejazi H3 ; Shirani D4 ; Momtaz H5
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Authors Affiliations
  1. 1. Department of Small Animal Internal Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
  2. 2. Department of Small Animal Internal Medicine, Clinical Science, University of Tehran, Tehran, Iran
  3. 3. Department of Parasitology and Mycology, School of Medicine, Esfahan University of Medical Science, Isfahan, Iran
  4. 4. Department of Clinical Science, University of Tehran, Tehran, Iran
  5. 5. Department of Microbiology, Shahrekord Branch, Azad University, Shahrekord, Iran

Source: Comparative Clinical Pathology Published:2013


Abstract

The present case-control study was conducted to determine the prevalence of Haemobartonella felis, study the role of its risk factors and compare the different diagnostic tests. In order to achieve these goals, direct blood smear and single PCR assay were performed on blood samples from 90 cats (45 healthy controls and 45 sick cats). In addition, age, sex, breed, outdoor access, castration and density of living area were recorded in each group, and haematological analysis was performed on all blood samples. A total of 50 and 72.2 % of cats were H. felis positive in direct blood smear and PCR, respectively. The results showed a significant difference between control and case groups regarding the quantitative variables including fever and dehydration in direct blood smear, and tachycardia, icterus, fever and dehydration in PCR (P < 0.05). Castration, sex, breed, access to the outside (lifestyle) and density of cat population did not significantly affect test results. The results indicate that interactive effects of various factors (risk factors of infection) can diminish the individual specific effect of each. © 2012 Springer-Verlag London Limited.
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