Computer-Aided Detection of Proliferative Cells and Mitosis Index in Immunohistichemically Images of Meningioma Publisher



Anari V¹ ; Mahzouni P² ; Amirfattahi R³ Show Affiliations
Source: 2010 6th Iranian Conference on Machine Vision and Image Processing, MVIP 2010 Published:2010

Abstract

Immuonohistochemically images of meningioma which are stained by ki67 marker contain positive and negative cells. Accurate counting the number of positive and negative cells in such images play a critical role in diagnosing diffrent type of meningioma cancer. Since pathological images of meningioma contain complex cell cluster accurate cell counting methodology is a major challenge for pathologist physicians. In this paper we provide a computer aided algorithm for detecting proliferative cells and mitosis index in immunohistochemically images of meningioma. In the first stage of the algorithm fuzzy c-means clustering was used to extract positive and negative cells based on CIElab color space. In the second stage, ultraerosion operation was applied to count the number of individual and overlapped cells. Experimental result show that the proposed algorithm is able to overcome some disadvantage of traditional approaches with acceptable accuracy by pathologist physicians. © 2010 IEEE.