Pd-1/Pd-L1 Interaction Regulates Bcl2, Ki67, Bax, and Casp3, Altering Proliferation, Survival, and Apoptosis in Acute Myeloid Leukemia Publisher Pubmed



Soltani M¹ ; Vosoughi M¹ ; Ganjalikhanihakemi M^{1, 2} ; Shapoorian H¹ ; Beshkar P³ ; Eskandari N¹ ; Ghezelbash B¹ Show Affiliations
Source: Iranian Journal of Allergy, Asthma and Immunology Published:2023

Abstract

Programmed death ligand-1 (PD-L1) is a pivotal inhibitory checkpoint ligand known to induce T-cell exhaustion via interaction with the programmed death‑1 (PD‑1) receptor. Beyond this, PDL1’s intrinsic signaling pathways within cancer cells warrant further exploration. This study aims to elucidate the effect of PD-L1 stimulation on the proliferation, survival, and apoptosis of acute myeloid leukemia (AML) cell lines. Two human AML cell lines, HL-60 and THP-1 were cultured and treated with phorbol 12-myristate 13-acetate (PMA) to induce PD-L1overexpression. Post-treatment PD-L1 expression was confirmed via flow cytometry. Subsequently, cell surface PD-L1 was stimulated using a recombinant PD-1, 24 hours post-PMA treatment. The expression alterations in pivotal genes including BCL2, MKI67, BAX, and CASP3 were monitored using quantitative real-time polymerase chain reaction 24 and 48 hours post-treatment. Additionally, annexin-V through flow cytometry. Findings reveal that PD-L1 stimulation augments AML cell proliferation and survival by enhancing MKI67 and BCL2 expressions while concurrently inhibiting cell apoptosis due to decreased BAX and CASP3 expression following PD-L1 stimulation. Notably, stimulated cells expressed exhibited reduced annexin-V compared to control cells. This study underscores that PD-L1 stimulation fosters AML cell proliferation and survival while impeding cell apoptosis. The results hold potential implications for targeting PD-L1 in AML treatment strategies. Copyright © 2023 Soltani et al. Published by Tehran University of Medical Sciences.