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A3 Adenosine Receptor Agonist Induce G1 Cell Cycle Arrest Via Cyclin D and Cyclin-Dependent Kinase 4 Pathways in Ovcar-3 and Caov-4 Cell Lines Publisher Pubmed



Joshaghani H1 ; Jafari S2, 3 ; Aghaei M3, 4 ; Panjehpour M3, 5 ; Abedi H3
Authors
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Authors Affiliations
  1. 1. Medical Laboratory Research Center, Golestan University of Medical Sciences, Gorgan, Iran
  2. 2. Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, Iran
  3. 3. Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, P.O. Box: 81746-73461, Isfahan, Iran
  4. 4. Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Iran
  5. 5. Bioinformatics Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Cancer Research and Therapeutics Published:2017


Abstract

Aim of the Study: The cell cycle, a vital process that involves in cells' growth and division, lies at the heart of cancer. It has been shown that IB-MECA, an A3 adenosine receptor agonist inhibits the proliferation of cancer cells by inducing cell cycle arrest in several tumors. In this study, we evaluated the role of IB-MECA inhibition in cell cycle progression in ovarian cancer cells. Materials and Methods: Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay in Caov-4 and OVCAR-3. Analysis of cell cycle distribution was carried out by flow cytometry. To determine the mechanisms of IB-MECA-mediated induction of cell cycle arrest, the expression of cell cycle regulatory proteins Cyclin D1 and cyclin-dependent kinase 4 (CDK4) was evaluated. Results: Our results showed that IB-MECA significantly reduced cell viability in a dose-dependent manner. Moreover, our results indicated that a low concentration of IB-MECA induced G1 cell cycle arrest. Reduction of Cyclin D1 and CDK4 protein levels was also observed after treating cancer cells with IB-MECA. Conclusion: This study demonstrated that IB-MECA induces G1 phase cell cycle arrest through Cyclin D1/CDK4-mediated pathway in ovarian cancer cells. © 2017 Journal of Cancer Research and Therapeutics.
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