Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
The Effect of Orexin-2 and Endocannabinoid-1 Antagonists on Neuronal Activity of Hippocampal Ca1 Pyramidal Neurons in Response to Tramadol in Rats Publisher



Imanpour V1 ; Reisi P1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Advanced Biomedical Research Published:2022


Abstract

Background: CA1, as a major structure involved in learning and memory, has been shown to be affected by tramadol addiction. Both orexin and endocannabinoid receptors express in CA1 and play an important role in drug dependency. The aim of this study was to evaluate the modulatory effects of orexin-2 (OX2R) and endocannabinoid-1 (CB1R) receptors on neuronal activity in CA1, in response to tramadol in rats. Materials and Methods: Male Wistar rats were divided into 8 groups (n = 6-7); saline-dimethyl sulfoxide (DMSO), tramadol-DMSO, saline-TCS-OX2-29, saline-AM251, tramadol-TCS-OX2-29, tramadol-AM251, saline-TCS-OX2-29-AM251, tramadol-TCS-OX2-29-AM251. Tramadol was injected intraperitoneally, and then, AM251 (1 nmol/0.3 µL), CB1R antagonist and TCS-OX2-29 (1 nmol/0.3 µL), OX2R antagonist, were microinjected individually or concurrently into the CA1. Using in vivo extracellular single-unit recording, the firing of CA1 pyramidal neurons was investigated. Results: Tramadol decreased neuronal activity in CA1 (P < 0.01) but increased it after micro-injection of DMSO. TCS-OX2-29 increased neuronal activity in saline group (P < 0.05) but decreased it in tramadol group. AM251 had no effect on saline group but decreased neuronal activity in tramadol group (P < 0.05). Concurrent micro-injection of TCS-OX2-29 and AM251 had no effect on saline group but decreased neuronal activity in tramadol group (P < 0.05). Conclusions: Our findings suggest that neural activity in CA1 is rapidly affected by acute use of tramadol, and some of these effects may be induced through the endocannabinoid and orexin systems. Thus, the function of endocannabinoid and orexin systems in CA1 may play a role in tramadol addiction. © 2022 Advanced Biomedical Research | Published by Wolters Kluwer-Medknow.
Related Docs
View other Related Docs
1. Effects of Selective Orexin Receptor-2 and Cannabinoid Receptor-1 Antagonists on the Response of Medial Prefrontal Cortex Neurons to Tramadol, Synapse (2022)
2. Mutual Assistance of Nucleus Accumbens Cannabinoid Receptor-1 and Orexin Receptor-2 in Response to Nicotine: A Single-Unit Study, Research in Pharmaceutical Sciences (2021)
3. The Acute Effects of Different Doses of Tramadol on Neuronal Activity of Medial Prefrontal Cortex in Rats, Advanced Biomedical Research (2022)
4. Functional Crosstalk of Nucleus Accumbens Cb1 and Ox2 Receptors in Response to Nicotine-Induced Place Preference, Neuroscience Letters (2019)
5. Electrophysiological Study of the Response of Ventral Tegmental Area Non-Dopaminergic Neurons to Nicotine After Concurrent Blockade of Orexin Receptor-2 and Cannabinoid Receptors-1, Brain Research (2019)
6. Effects of Concurrent Blockade of Ox2 and Cb1 Receptors in the Ventral Tegmental Area on Nicotine-Induced Place Preference in Rats, Neuroscience Letters (2018)
7. Orexin Type-2 Receptor Blockade Prevents the Nicotine-Induced Excitation of Nucleus Accumbens Core Neurons in Rats: An Electrophysiological Perspective, Pharmacological Reports (2019)
8. The Effect of Am281, a Cannabinoid Antagonist, on Memory Performance During Spontaneous Morphine Withdrawal in Mice, Research in Pharmaceutical Sciences (2013)
Experts (# of related papers)
View all Related Experts
Hojatallah Alaei (14)
Parham Reisi (9)
Other Related Docs
9. The Effect of Nimodipine on Memory Impairment During Spontaneous Morphine Withdrawal in Mice: Corticosterone Interaction, European Journal of Pharmacology (2012)
10. Roles of the Nucleus Accumbens (Shell) in the Acquisition and Expression of Morphine-Induced Conditioned Behavior in Freely Moving Rats, International Journal of Preventive Medicine (2014)
11. Influences of Morphine on Cannabinoid Receptor 1, 2 Expression in Breast Cancer Cell Lines, International Journal of Women's Health and Reproduction Sciences (2024)
12. Blockade of Prelimbic Glutamate Receptor Reduces the Reinforcing Effect of Morphine, Canadian Journal of Physiology and Pharmacology (2018)
13. Neuronal Calcium Channels Blocker, Ziconotide (ɷ-Conotoxin Mviia), Reverses Morphine Withdrawal-Induced Memory Impairments Via Alteration in Hippocampal Nmda Receptor Expression in Rats, Toxin Reviews (2020)
14. The Effect of the Blockage of the Receptors of Glutamate N-Methyl-D-Aspartate (Nmda) in Prefrontal Cortex on Morphine Tendency in Male Rat, Journal of Isfahan Medical School (2016)
15. Electrical Stimulation Mpfc Affects Morphine Addiction by Changing Glutamate Concentration in the Ventral Tegmental Area, Metabolic Brain Disease (2019)
16. The Role of Medial Prefrontal Cortex and Ventral Tegmental Areas in Withdrawal Syndrome in Morphine Addicted Male Rats, Journal of Isfahan Medical School (2020)
17. The Cb1 Receptor Antagonist, Am281, Improves Recognition Loss Induced by Naloxone in Morphine Withdrawal Mice, Basic and Clinical Pharmacology and Toxicology (2012)
18. The Blockade of Glutamate N-Methyl-D-Aspartate Receptors Into the Prelimbic of Prefrontal Cortex Decreases Morphine-Induced Conditioned Place Preference in Rat, Indonesian Biomedical Journal (2017)
19. Different Current Intensities Electrical Stimulation of Prelimbic Cortex of Mpfc Produces Different Effects on Morphine-Induced Conditioned Place Preference in Rats, Behavioural Brain Research (2012)
20. Am281, Cannabinoid Antagonist/Inverse Agonist, Ameliorates Scopolamine-Induced Cognitive Deficit, Iranian Journal of Basic Medical Sciences (2012)
21. Acute and Chronic Tramadol Administration Impair Spatial Memory in Rat, Research in Pharmaceutical Sciences (2016)
22. The Effect of Nimodipine on Memory Loss Following Naloxone-Induced Morphine Withdrawal in Object Recognition, Research in Pharmaceutical Sciences (2014)
23. The Effects of Mid-Term Running Activity on Passive Avoidance Learning and Memory in Opioid Addicted Rats, Journal of Isfahan Medical School (2009)
24. Lateral Habenula Electrical Stimulation With Different Intensities in Combination With Gabab Receptor Antagonist Reduces Acquisition and Expression Phases of Morphine-Induced Cpp, Neuroscience Letters (2021)