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Component-Resolved Diagnosis of American Cockroach (Periplaneta Americana) Allergy in Patients From Different Geographical Areas Publisher



Wangorsch A1 ; Jamin A1 ; Eichhorn S2 ; Pablos I2 ; Sharma S3 ; Schweidler B2 ; Kastner B2 ; Wildner S2 ; Saloga J4 ; Fuhrer F5 ; Reyna Orozco RR6 ; Sherkat R7 ; Sadeghi S7 ; Teifoori F7 Show All Authors
Authors
  1. Wangorsch A1
  2. Jamin A1
  3. Eichhorn S2
  4. Pablos I2
  5. Sharma S3
  6. Schweidler B2
  7. Kastner B2
  8. Wildner S2
  9. Saloga J4
  10. Fuhrer F5
  11. Reyna Orozco RR6
  12. Sherkat R7
  13. Sadeghi S7
  14. Teifoori F7
  15. Park JW8
  16. Briza P2
  17. Vieths S1
  18. Ferreira F2
  19. Arora N3
  20. Lidholm J9
  21. Gadermaier G2
  22. Scheurer S1
Show Affiliations
Authors Affiliations
  1. 1. Molecular Allergology, Paul-Ehrlich-Institut, Langen, Germany
  2. 2. Department of Biosciences, Paris Lodron University of Salzburg, Salzburg, Austria
  3. 3. Allergy and Immunology Section, CSIR-Institute of Genomics and Integrative Biology, New Delhi, India
  4. 4. Department of Dermatology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany
  5. 5. Batch Control and Allergen Analysis, Paul-Ehrlich-Institut, Langen, Germany
  6. 6. Clinical Immunology Service in Hospital Militar Dr. Carlos Arvelo, Caracas, Venezuela
  7. 7. Acquired Immunodeficiency Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  8. 8. Department of Internal Medicine, Yonsei University College of Medicine, Seoul, South Korea
  9. 9. Thermo Fisher Scientific, Immunodiagnostics, Uppsala, Sweden

Source: Frontiers in Allergy Published:2021


Abstract

Background: Manifestation of respiratory allergy to American cockroach (Periplaneta americana) is prominent in the subtropical and tropical areas. However, co-existing perennial indoor inhalant allergies frequently compromise clinical diagnosis of cockroach allergy, and the analysis of sensitization pattern is limited by the lack of Periplaneta allergens widely available for component-resolved diagnostics (CRD). Objective: To evaluate a collection of previously described recombinant Periplaneta allergens for CRD in cockroach allergy. Methods: A panel of nine recombinant Periplaneta allergens (Per a 1–5, 7–10) was generated, purified, and subjected to physicochemical characterization by applying circular dichroism (CD) spectroscopy, dynamic light scattering (DLS), amino acid (AA) analysis, and mass spectrometry (MS). Patients (n = 117) from India, Korea, Venezuela, and Iran, reporting perennial respiratory indoor allergies with IgE sensitization to cockroach (P. americana and/or Blattella germanica), were included. The sensitization profile was monitored by the experimental ImmunoCAP testing. Results: ImmunoCAP testing confirmed IgE sensitization to Periplaneta and/or Blattella extract in 98 of 117 patients (r = 0.95). Five out of 117 patients were sensitized to only one of the two cockroach species. Within the whole study group, the prevalence of sensitization to individual allergens varied from 4% (Per a 2) to 50% (Per a 9), with the highest IgE values to Per a 9. Patients from four countries displayed different sensitization profiles at which Per a 3 and Per a 9 were identified as major allergens in India and Korea. Periplaneta-derived lipocalin and myosin light chain were characterized as new minor allergens, designated as Per a 4 and Per a 8. Periplaneta extract showed higher diagnostic sensitivity than all individual components combined, suggesting the existence of allergens yet to be discovered. Conclusion: Utilization of a panel of purified Periplaneta allergens revealed highly heterogeneous sensitization patterns and allowed the classification of lipocalin and myosin light chain from Periplaneta as new minor allergens. Copyright © 2021 Wangorsch, Jamin, Eichhorn, Pablos, Sharma, Schweidler, Kastner, Wildner, Saloga, Fuhrer, Reyna Orozco, Sherkat, Sadeghi, Teifoori, Park, Briza, Vieths, Ferreira, Arora, Lidholm, Gadermaier and Scheurer.