Comparison of Dna Instability in Adenoma and Adenocarcinoma in Patients With Colorectal Cancer, Using Random Amplified Polymorphic Dna Polymerase Chain Reaction (Rapd-Pcr)



Talebi A¹ ; Daneshbakhtyiar S¹ ; Meshkat M² ; Meshkat M²
Source: Journal of Isfahan Medical School Published:2017

Abstract

Background: Colorectal cancer is the consequence of gathering numerous genetic alterations and it has been suggested that genomic instability is indispensable for the generation of multiple mutations underlying the development of cancer. Methods: Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) method was utilized to find genomic alterations in adenomas and adenocarcinomas compared with normal epithelial tissue obtained from 17 patients with colorectal cancer. Findings: Separated PCR products by 2% agarose determined approximate 370 base pairs band as a polymorphic fingerprint for normal (11.8%), adenoma (76.5%), and adenocarcinoma (88.2%) tissues. Polymorphic band could significantly discriminate adenomas [Odds ratio (OR) = 24.38, P = 0.0004] and adenocarcinomas (OR = 56.25, P < 0.0001) from normal tissues. Furthermore, the 370 base pairs band could not distinguish adenomas from adenocarcinomas (P = 0.6600). Conclusion: Further investigations can be aimed for sequencing and disclosing functional outcome of gaining 370 base pairs band. © 2017, Isfahan University of Medical Sciences(IUMS). All rights reserved.