Recombinant Human Lipocalin 2 Acts As an Antibacterial Agent to Prevent Platelet Contamination Publisher Pubmed



Bakhshandeh Z¹ ; Halabian R² ; Fooladi AAI² ; Jahaniannajafabadi A³ ; Jalili MA¹ ; Roudkenar MH¹ Show Affiliations
Source: Hematology Published:2014

Abstract

Background: Bacterial contamination of platelet products is the major infectious risk in blood transfusion medicine, which can result in life-threatening sepsis in recipient. Lipocalin 2 (Lcn2) is an iron-sequestering protein in the antibacterial innate immune response, which inhibit bacterial growth. This study was aimed to evaluate the antibacterial property of Lcn2 in preventing bacterial contamination of platelets.; Methods: Recombinant Lcn2 was expressed in a eukaryotic expression system and following purification and characterization of the recombinant Lcn2, its minimum inhibitory concentration was determined. Then, platelet concentrates were inoculated with various concentrations of Staphylococcus epidermidis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, and Enterococcus faecalis, and the antibacterial effects of Lcn2 was evaluated at 20–24°C.; Results: Results revealed that Lcn2 effectively inhibited the growth of 1.5 × 104 CFU/ml S. epidermidis, P. aeruginosa, K. pneumoniae, E. coli, and E. faecalis at 40 ng/ml. At this concentration, Lcn2 also inhibited the growth of 1.5 × 103 CFU/ml Staphylococcus aureus and Proteus mirabilis.; Conclusion: Recombinant Lcn2 inhibited growth of a variety of platelet-contaminating bacteria. Therefore, supplementation of platelet concentrates with Lcn2 may reduce bacterial contamination. © W. S. Maney & Son Ltd 2014.