Search for Mhc-Associated Genes in Human: Five New Genes Centromeric to Hla-Dp With yet Unknown Functions Publisher Pubmed



Janatipour M^{1, 2} ; Naumov Y¹ ; Ando A¹ ; Sugimura K¹ ; Okamoto N¹ ; Tsuji K¹ ; Abe K³ ; Inoko H¹
Source: Immunogenetics Published:1992

Abstract

Taking advantage of five mouse genomic or cDNA probes [KE5(probe 14), KE4 (probe11), KE3 (probe7), KE2 (probe5), and SET] mapped on the H-2K region in mouse, we have identified and localized homologues of these five genes in the human major histocompatibility complex region (HKE5, HKE4, HKE3, HKE2, and HSET, respectively). Cosmid cloning and pulsed field gel electrophoresis analyses indicated that a human homologous gene, HKE5, is located 10 kilobases (kb) centromeric of the α2(XI) collagen (COL11A2) gene followed by HKE4. HKE3, closely linked to HKE2, is located 170 kb centromeric of HKE4. Furthermore, HSET is located 50 kb centromeric of HKE2. This gene organization outside the DP subregion is completely identical to that of the mouse H-2K region centromeric of I-Pb 3, a mouse homologue of the DPB gene, except the lack of genes corresponding to the H-2K and -K2 genes in human. © 1992 Springer-Verlag.