Investigation of a Medicinal and Ornamental Plant Propagation, the Cereus Peruvianus Mill. and Using Tissue Culture Technique

Investigation of a Medicinal and Ornamental Plant Propagation, the Cereus Peruvianus Mill. and Using Tissue Culture Technique

Karimi N¹ ; Naderi R² ; Ebrahimi M³ ; Mofid MR⁴

Show Affiliations

1. Faculty of Horticultural Sciences, Islamic Azad University (Karaj Branch), Karaj, Iran
2. Faculty of Horticultural Sciences, University of Tehran, Karaj, Iran
3. Agriculture Biotechnology Institute of Iran-Central Region, Isfahan, Iran
4. Department of Biochemistry, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Medicinal Plants Published:2010

Abstract

Background: The extracted juice and alkaloeids of the medicinal and ornamental cactus Cereus peruvianus are contain a lot of effective compounds which widely use in medicine and agricultural industries. Propagation of this plant using conventional method (seed, cutting, offset) is very slow and its mass production is possible by tissue culture technique. Objective: The aim of this study was the micropropagation possibility and recognition of some of its different aspects, and determination of suitable medium and hormonal composition at invitro conditions of cereus. Methods: The experimental design was compeletly randomized with 3 replication and each replicate was contained 5 sample. After surface disinfection the explants were cultured on MS basal medium containing %3 sucrose,8 g/l phytoagar and different treatment of plant growth regulators (IBA, NAA, Kinetin, BAP, 2,4-D, GA3, TDZ) and pH=5/8. After 45 days, some parameters such as effectivness of culture media in percent of regeneration from areole, production of friable callus and rooting in all treatments were recorded. Results: In this research The best growth of friable callus was obtained in MS medium supplemented with 0.07mg/l TDZ and 0.05 mg/l NAA and 4mg/l 2,4-D with 6mg/l kinetin for regeneration of areole and 4mg/l 2,4-D and 4mg/l kinetin for rooting was the most favorable medium.High regeneration potential was observed after 2.5-3 months of culture. Eight different media was selected for plantlet regeneration.There is a significant difference between regeneration of areole in cereus peruvianus var monstrosus and cereus peruvianus var tortuosus. Conclusion: As for to the importance of the extracted juice and alkaloeids of the cactus Cereus peruvianus Propagation of this plant using by conventional method is very slow and propagation it of areol in addition to economical is very fast.

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Mohammad Reza Mofid (2)

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Style	Citing Format
MLA	Karimi N, et al.. "Investigation of a Medicinal and Ornamental Plant Propagation, the Cereus Peruvianus Mill. and Using Tissue Culture Technique." Journal of Medicinal Plants, vol. 9, no. 34, 2010, pp. 38-45.
APA	Karimi N, Naderi R, Ebrahimi M, Mofid MR (2010). Investigation of a Medicinal and Ornamental Plant Propagation, the Cereus Peruvianus Mill. and Using Tissue Culture Technique. Journal of Medicinal Plants, 9(34), 38-45.
Chicago	Karimi N, Naderi R, Ebrahimi M, Mofid MR. "Investigation of a Medicinal and Ornamental Plant Propagation, the Cereus Peruvianus Mill. and Using Tissue Culture Technique." Journal of Medicinal Plants 9, no. 34 (2010): 38-45.
Harvard	Karimi N et al. (2010) 'Investigation of a Medicinal and Ornamental Plant Propagation, the Cereus Peruvianus Mill. and Using Tissue Culture Technique', Journal of Medicinal Plants, 9(34), pp. 38-45.
Vancouver	Karimi N, Naderi R, Ebrahimi M, Mofid MR. Investigation of a Medicinal and Ornamental Plant Propagation, the Cereus Peruvianus Mill. and Using Tissue Culture Technique. Journal of Medicinal Plants. 2010;9(34):38-45.
BibTex	@article{ author = {Karimi N and Naderi R and Ebrahimi M and Mofid MR}, title = {Investigation of a Medicinal and Ornamental Plant Propagation, the Cereus Peruvianus Mill. and Using Tissue Culture Technique}, journal = {Journal of Medicinal Plants}, volume = {9}, number = {34}, pages = {38-45}, year = {2010} }
RIS	TY - JOUR AU - Karimi N AU - Naderi R AU - Ebrahimi M AU - Mofid MR TI - Investigation of a Medicinal and Ornamental Plant Propagation, the Cereus Peruvianus Mill. and Using Tissue Culture Technique JO - Journal of Medicinal Plants VL - 9 IS - 34 SP - 38 EP - 45 PY - 2010 ER -

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