Monoclonal Antibody Against Ror1 Induces Apoptosis in Human Bladder Carcinoma Cells

Monoclonal Antibody Against Ror1 Induces Apoptosis in Human Bladder Carcinoma Cells

Bayat AA¹ ; Sadeghi N¹ ; Fatemi R¹ ; Nowroozi MR² ; Moghadam SO² ; Borzuee M² ; Radmanesh A³ ; Khodadoost M⁴ ; Sarrafzadeh AR⁵ ; Zarei O⁶ ; Rabbani H¹

Show Affiliations

1. Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
2. Uro-Oncology Research Center, Tehran University of Medical Sciences, Tehran, Iran
3. Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran
4. Faculty of Traditional Medicine, Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5. Department of Pathology, Khatam Al Anbia Hospital, Tehran, Iran
6. Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran

Source: Avicenna Journal of Medical Biotechnology Published:2020

Abstract

Background: Receptor tyrosine kinase-like Orphan Receptor 1 (ROR1) is one of the promising cell surface antigens for targeting cancer cells. The aim of this study was to evaluate ROR1 cell surface expression in bladder cancer cells using a murine anti-ROR1 monoclonal antibody (mAb) called 5F1-B10 as well as investigate its potential in apoptosis induction. Methods: Expression of ROR1 in two human bladder cell lines, 5637 and EJ138, as well as a non-cancerous human cell line, Human Fetal Foreskin Fibroblast (HFFF), was examined by flow cytometry and immunocytochemistry. Immunohistochemical staining of cancer and normal bladder tissues was also performed. Results: The flow cytometry results showed that 5F1-B10 mAb could recognize ROR1 molecules in 86.1% and 45.6% of 5637 and EJ138 cells, respectively. The expression level of ROR1 was 5.49% in HFFF cells. The immunocytochemistry and immunohistochemistry staining results also confirmed the presence of ROR1 on the surface of both bladder cancer cells and tissues, respectively. The obtained data from apoptosis assay demonstrated that 5F1-B10 mAb could induce apoptosis in both 5637 and EJ138 cell lines. Conclusion: Taken together, our finding indicates the role of ROR1 in bladder cancer cell survival and suggests this receptor might be a promising target for developing novel therapeutic agents against bladder carcinoma. © 2020, Avicenna Journal of Medical Biotechnology.

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Style	Citing Format
MLA	Bayat AA, et al.. "Monoclonal Antibody Against Ror1 Induces Apoptosis in Human Bladder Carcinoma Cells." Avicenna Journal of Medical Biotechnology, vol. 12, no. 3, 2020, pp. 165-171.
APA	Bayat AA, Sadeghi N, Fatemi R, Nowroozi MR, Moghadam SO, Borzuee M, Radmanesh A, Khodadoost M, Sarrafzadeh AR, Zarei O, Rabbani H (2020). Monoclonal Antibody Against Ror1 Induces Apoptosis in Human Bladder Carcinoma Cells. Avicenna Journal of Medical Biotechnology, 12(3), 165-171.
Chicago	Bayat AA, Sadeghi N, Fatemi R, Nowroozi MR, Moghadam SO, Borzuee M, Radmanesh A, et al.. "Monoclonal Antibody Against Ror1 Induces Apoptosis in Human Bladder Carcinoma Cells." Avicenna Journal of Medical Biotechnology 12, no. 3 (2020): 165-171.
Harvard	Bayat AA et al. (2020) 'Monoclonal Antibody Against Ror1 Induces Apoptosis in Human Bladder Carcinoma Cells', Avicenna Journal of Medical Biotechnology, 12(3), pp. 165-171.
Vancouver	Bayat AA, Sadeghi N, Fatemi R, Nowroozi MR, Moghadam SO, Borzuee M, et al.. Monoclonal Antibody Against Ror1 Induces Apoptosis in Human Bladder Carcinoma Cells. Avicenna Journal of Medical Biotechnology. 2020;12(3):165-171.
BibTex	@article{ author = {Bayat AA and Sadeghi N and Fatemi R and Nowroozi MR and Moghadam SO and Borzuee M and Radmanesh A and Khodadoost M and Sarrafzadeh AR and Zarei O and Rabbani H}, title = {Monoclonal Antibody Against Ror1 Induces Apoptosis in Human Bladder Carcinoma Cells}, journal = {Avicenna Journal of Medical Biotechnology}, volume = {12}, number = {3}, pages = {165-171}, year = {2020} }
RIS	TY - JOUR AU - Bayat AA AU - Sadeghi N AU - Fatemi R AU - Nowroozi MR AU - Moghadam SO AU - Borzuee M AU - Radmanesh A AU - Khodadoost M AU - Sarrafzadeh AR AU - Zarei O AU - Rabbani H TI - Monoclonal Antibody Against Ror1 Induces Apoptosis in Human Bladder Carcinoma Cells JO - Avicenna Journal of Medical Biotechnology VL - 12 IS - 3 SP - 165 EP - 171 PY - 2020 ER -

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