Pten Promoter Methylation and Expression in Endometrial Cancer Tissues

Pten Promoter Methylation and Expression in Endometrial Cancer Tissues Publisher Pubmed

Khatami F¹ ; Shahriari S² ; Aminimoghaddam S³ ; Klashami ZN¹ ; Farahani MS⁴ ; Teimooritoolabi L⁵ ; Amoli MM¹ ; Asadi M⁴ ; Rashidi BH⁶

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1. Urology Research Center, Tehran University of Medical Sciences, Tehran, Iran
2. Metabolic Disorders Research Centre, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Obstetrics and Gynecology, Iran University of Medical Sciences, Tehran, Iran
4. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
5. Molecular Medicine Department, Pasteur Institute of Iran, Tehran, Iran
6. Vali-e Asr Reproductive Health Research Center, Tehran University of Medical Sciences, Tehran, Iran

Source: Epigenomics Published:2023

Abstract

Introduction: Some gene expression regulation in cancers can be controlled by epigenetic change like methylation. PTEN promoter methylation and expression were evaluated in endometrial cancer. Methods: The study was run on 39 tumor tissues of endometrial cancer patients and 41 normal endometrial tissues. After total RNA extraction, cDNA synthesis was done by reverse transcription of the total (real-time PCR) using SYBER Green master mix. DNA extraction and bisulfite treatment were conducted and methylation was semiquantified by the methylation-sensitive high-resolution melting method. Finally, promoter methylation quantification of the total number of 25 tumors and 22 non-neoplastic tissues was done. Results: PTEN gene expression showed a significant decrease in endometrial cancer tissues. Promoter methylation was significantly lower in the non-neoplastic group (7.2; p < 0.001). In addition, PTEN promoter methylation was observed in 52.0% of tumor tissues compared with 13.6% in the non-neoplastic group (p = 0.06). There were no significant correlations between PTEN expression and methylation and clinicopathological features in endometrial cancer patients (p > 0.05). Conclusion: PTEN gene expression in endometrial cancer tissues decreased because of its promoter hypermethylation. © 2023 Future Medicine Ltd.

Style	Citing Format
MLA	Khatami F, et al.. "Pten Promoter Methylation and Expression in Endometrial Cancer Tissues." Epigenomics, vol. 15, no. 8, 2023, pp. 507-516.
APA	Khatami F, Shahriari S, Aminimoghaddam S, Klashami ZN, Farahani MS, Teimooritoolabi L, Amoli MM, Asadi M, Rashidi BH (2023). Pten Promoter Methylation and Expression in Endometrial Cancer Tissues. Epigenomics, 15(8), 507-516.
Chicago	Khatami F, Shahriari S, Aminimoghaddam S, Klashami ZN, Farahani MS, Teimooritoolabi L, Amoli MM, Asadi M, Rashidi BH. "Pten Promoter Methylation and Expression in Endometrial Cancer Tissues." Epigenomics 15, no. 8 (2023): 507-516.
Harvard	Khatami F et al. (2023) 'Pten Promoter Methylation and Expression in Endometrial Cancer Tissues', Epigenomics, 15(8), pp. 507-516.
Vancouver	Khatami F, Shahriari S, Aminimoghaddam S, Klashami ZN, Farahani MS, Teimooritoolabi L, et al.. Pten Promoter Methylation and Expression in Endometrial Cancer Tissues. Epigenomics. 2023;15(8):507-516.
BibTex	@article{ author = {Khatami F and Shahriari S and Aminimoghaddam S and Klashami ZN and Farahani MS and Teimooritoolabi L and Amoli MM and Asadi M and Rashidi BH}, title = {Pten Promoter Methylation and Expression in Endometrial Cancer Tissues}, journal = {Epigenomics}, volume = {15}, number = {8}, pages = {507-516}, year = {2023} }
RIS	TY - JOUR AU - Khatami F AU - Shahriari S AU - Aminimoghaddam S AU - Klashami ZN AU - Farahani MS AU - Teimooritoolabi L AU - Amoli MM AU - Asadi M AU - Rashidi BH TI - Pten Promoter Methylation and Expression in Endometrial Cancer Tissues JO - Epigenomics VL - 15 IS - 8 SP - 507 EP - 516 PY - 2023 ER -

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