Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
The Role of Long Non-Coding Rna Linc00839 in Oral Squamous Cell Carcinoma Based on Bioinformatics and Experimental Research Publisher Pubmed



Kalantarycharvadeh A1, 2 ; Morovat S3 ; Aslani S1 ; Ziamajidi N1 ; Emami Razavi A4 ; Abbasalipourkabir R1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Clinical Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
  2. 2. Student Research Committee, Hamadan University of Medical Sciences, Hamadan, Iran
  3. 3. Department of Medical Genetics and Molecular Biology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
  4. 4. Iran National Tumor Bank, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran

Source: Scientific Reports Published:2024


Abstract

This study explores the role of LINC00839 and its potential interaction with the miR-195-5p/cyclin E1 (CCNE1) axis in oral squamous cell carcinoma (OSCC). Using The Cancer Genome Atlas, we analyzed lncRNA, miRNA, and mRNA sequencing data for OSCC. Different online tools were applied to detect lncRNA-related miRNAs and their target mRNAs, forming a lncRNA/miRNA/mRNA axis. Co-expression analysis determined the correlation between lncRNA and mRNA expression. Afterward, protein-protein interaction network and functional enrichment analyses disclosed the biological activity of target genes. The expression and correlations of LINC00839, miR-195-5p, and CCNE1 were examined in 30 pairs of OSCC and noncancerous tissues. A Chi-square test was used to determine clinicopathological associations, and ROC analysis estimated diagnostic value. A total of 66 differentially expressed lncRNAs, 80 miRNAs, and 1149 mRNAs were identified in OSCC versus non-tumor samples. After filtering lncRNAs based on novelty, and predicting lncRNA-miRNA, and miRNA-mRNA interactions, the LINC00839/miR-195-5p/CCNE1 axis was discovered. RT-qPCR showed upregulation of LINC00839 and CCNE1 was accompanied by the downregulation of miR-195-5p. A significant positive correlation was observed between LINC00839 and CCNE1 mRNA expression, along with a significant negative correlation between LINC00839 and miR-195-5p expression. Moreover, increased LINC00839 was associated with tumor grade and lymph node status, while decreased miR-195-5p was correlated with lymph, depth, and vascular invasion (p < 0.05). The combined ROC curve demonstrated a significant area under the curve of 0.93. This discovery reveals a novel regulatory mechanism underlying OSCC tumorigenesis and may provide effective diagnosis and potential therapeutic targets to cure this devastating cancer. © The Author(s) 2024.
Related Docs
View other Related Docs
1. Micrornas-Mediated Regulation of the Differentiation of Dental Pulp-Derived Mesenchymal Stem Cells: A Systematic Review and Bioinformatic Analysis, Stem Cell Research and Therapy (2023)
2. Rewiring of Mirna-Mrna Bipartite Co-Expression Network As a Novel Way to Understand the Prostate Cancer Related Players, Systems Biology in Reproductive Medicine (2023)
3. Identification of Key Lncrnas Associated With Oxaliplatin Resistance in Colorectal Cancer Cells and Isolated Exosomes: From In-Silico Prediction to In-Vitro Validation, PLoS ONE (2024)
4. Mir-17-92A-1 Cluster Host Gene: A Key Regulator in Colorectal Cancer Development and Progression, Clinical and Experimental Medicine (2024)
5. Construction of Circrna-Mirna-Mrna Network in the Pathogenesis of Recurrent Implantation Failure Using Integrated Bioinformatics Study, Journal of Cellular and Molecular Medicine (2022)
6. Epigenetic Regulation in Oral Squamous Cell Carcinoma Microenvironment: A Comprehensive Review, Cancers (2023)
7. Identification of Antisense and Sense Rnas of Intracrine Fibroblast Growth Factor Components As Novel Biomarkers in Colorectal Cancer and in Silico Studies for Drug and Nanodrug Repurposing, Environmental Research (2023)
8. Non-Coding Rnas in Leukemia Drug Resistance: New Perspectives on Molecular Mechanisms and Signaling Pathways, Annals of Hematology (2024)
Experts (# of related papers)
View all Related Experts
Zahra Salehi (2)
Mohammad Vaezi (1)
Other Related Docs
9. Expression Analysis of Long Non-Coding Rnas Related With Foxm1, Gata3, Foxa1 and Esr1 in Breast Tissues, Frontiers in Oncology (2021)
10. Deciphering Crucial Genes in Coeliac Disease by Bioinformatics Analysis, Autoimmunity (2020)
11. Circrna-Associated Cerna Networks (Circcenets) in Chronic Obstructive Pulmonary Disease (Copd), Life Sciences (2024)
12. Tracing Vitamins on the Long Non-Coding Lane of the Transcriptome: Vitamin Regulation of Lncrnas, Genes and Nutrition (2024)
13. Germline Biallelic Mutation Affecting the Transcription Factor Helios Causes Pleiotropic Defects of Immunity, Science Immunology (2021)
14. Guca2a Dysregulation As a Promising Biomarker for Accurate Diagnosis and Prognosis of Colorectal Cancer, Clinical and Experimental Medicine (2024)
15. The Landscape of Non-Coding Rnas in the Immunopathogenesis of Endometriosis, Frontiers in Immunology (2023)
16. Identification of Lncpvt1 and Circpvt1 As Prognostic Biomarkers in Human Colorectal Polyps, Scientific Reports (2023)
17. Integrated Analysis of the Genetic Basis of Suicidal Behavior: What Has Been Shown by Structural Genetic Studies so Far, Psychiatric Genetics (2018)
18. Deciphering the Role of Loc124905135-Related Non-Coding Rna Cluster in Human Cancers: A Comprehensive Review, Heliyon (2024)
19. Unraveling Cancer Progression in Oral Squamous Cell Carcinoma Through Regulatory Network Analysis; Mirna-Target Gene Interaction, Molecular Biology (2024)
20. Cross-Talk Between Non-Coding Rnas and Pi3k/Akt/Mtor Pathway in Colorectal Cancer, Molecular Biology Reports (2021)
21. Heterogeneous Expression of Long Noncoding Rna Rp11-109D20.2: Insights Into Regulatory Gene Expression Roles in Colon Cancer, Iranian Journal of Basic Medical Sciences (2025)
22. Differential Expression Analysis in Epithelial Ovarian Cancer Using Functional Genomics and Integrated Bioinformatics Approaches, Informatics in Medicine Unlocked (2023)
23. Bcl11a and the Correlated Key Genes Ascribable to Globin Switching: An In-Silico Study, Cardiovascular and Hematological Disorders - Drug Targets (2022)
24. Systems Toxicology Assessment Revealed the Impact of Graphene-Based Materials on Cell Cycle Regulators, Journal of Biomedical Materials Research - Part A (2020)
25. The Role of Curcumin in Modulating Circular Rnas and Long Non-Coding Rnas in Cancer, Clinical and Translational Oncology (2025)
26. The Hsa-Mir-3613–5P, a Potential Oncogene Correlated With Diagnostic and Prognostic Merits in Kidney Renal Clear Cell Carcinoma, Pathology Research and Practice (2023)
27. Prdm12 Is Transcriptionally Active and Required for Nociceptor Function Throughout Life, Frontiers in Molecular Neuroscience (2021)
28. An Overview of Long Noncoding Rnas: Biology, Functions, Therapeutics, Analysis Methods, and Bioinformatics Tools, Cell Biochemistry and Function (2022)
29. Constructing Mrna, Mirna, Circrna and Lncrna Regulatory Network by Analysis of Microarray Data in Breast Cancer, Gene Reports (2022)
30. Immune Checkpoints and Ncrnas: Pioneering Immunotherapy Approaches for Hematological Malignancies, Cancer Cell International (2024)