Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Application of Electrospun Gelatin Nanofibers in Tissue Engineering



Naghibzadeh M1 ; Firoozi S2 ; Nodoushan FS3 ; Adabi M4 ; Khoradmehr A3 ; Fesahat F3 ; Esnaashari SS5 ; Khosravani M5 ; Adabi M4 ; Tavakol S7 ; Pazokitoroudi H8 ; Adel M9, 10 ; Zahmatkeshan M11
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Nanotechnology, Research and Clinical Centre for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
  2. 2. Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Stem Cell Biology Research Centre, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
  4. 4. Young Researchers and Elite Club, Roudehen Branch, Islamic Azad University, Roudehen, Iran
  5. 5. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Student's Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
  8. 8. Physiology Department and Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran
  9. 9. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tabriz University of Medical Sciences, Tehran, Iran
  10. 10. Deputy of research and technology, School of medicine, Iran University of Medical Sciences, Tehran, Iran
  11. 11. Neuroscience Research Center, Iran University of Medical Sciences, Tehran, Iran

Source: Biointerface Research in Applied Chemistry Published:2018

Abstract

The aim of this work was to prepare gelatin nanofibers and investigate their medical applications such as nanofibrous scaffolding for skin regeneration, and skin wound dressing in tissue engineering. First, gelatin nanofibers were prepared via electrospinning method. The morphology and diameter of the nanofibers were characterized by scanning electron microscopy (SEM).Based on the mechanical weakness of gelatin nanofibers, the heat treatment process was used, and the effects were investigated via mechanical tests. The results of the stress strain curve demonstrated that the mechanical properties of the nanofibers enhanced after heat treatment.The heat treated electrospun gelatin nanofibers were applied as scaffold, and human foreskin fibroblast cells (HFFs) were cultured on the scaffold and characterized by SEM. The results showed that the HFFs were completely spread on the surface of the scaffold. In addition, the toxicity of the scaffold was investigated using MTT assay. The scaffold exhibited desired biocompatibility and no toxicity after 24 and 48 hours in culture. In the other ways, the gelatin nanofibers were investigated as a wound dressing in vivo. The certain amount of Myrtus Communis essential oil was loaded in gelatin nanofibers and was used as skin care dressing in vivo. The results of this study indicated no positive effects of Myrtus Communis essential oil gelatin nanofibers (MEG) on the healing of the wound. © 2018 by the authors.