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Application of Free and Immobilized Laccase for Removal and Detoxification of Fluoroquinolones From Aqueous Solution Publisher



Ashrafi SD1 ; Nasseri S2, 3 ; Alimohammadi M2 ; Mahvi AH2, 3, 4, 5 ; Faramarzi MA6
Authors
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Authors Affiliations
  1. 1. Department of Environmental Health Engineering, Research Center of Health and Environment, School of Health, Guilan University of Medical Sciences, Rasht, Iran
  2. 2. Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Center for Water Quality Research (CWQR), Institute for Environmental Research (IER), Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Center for Solid Waste Research (CSWR), Institute for Environmental Research (IER), Tehran University of Medical Sciences, Tehran, Iran
  5. 5. National Institute of Health Research, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, 14174, Iran

Source: Global Nest Journal Published:2020


Abstract

Laccase from Trametes versicolor was immobilized by covalent bonds formation on CPC silica carriers. Elimination of two floroqinolone (FQ); enrofloxacine (ENR) and flumequine (FLU) using laccase in both free and immobilized form in the absence and presence of 1-hydroxybenzotriazole (HBT) and 4-Hydroxybenzoic acid (HBA) as mediators was investigated. Temperature, pH and storage stability of immobilized laccase was significantly improved compare to free laccase. In the absence of a laccase mediator, the initial concentrations of 50 mg L−1 of ENR and FLU decreased by 19 % and 28 %, respectively, after 6 h treatment using the immobilized laccase, while, the removal percentages were increased to 98 % and 96 %, respectively, when the immobilized laccase was used in presence of HBT. Whereas the removal percentages of ENR and FLU were increased to 97 % and 88 %, respectively, when the immobilized laccase was used in presence of HBA. After twenty runs of the enzymatic elimination (laccase-HBT system) of ENR and FLU, the immobilized laccase exhibited the relative removal of 17.63 % and 15.62 %, respectively. The results of microtoxicity test (growth inhabitation percentage of six bacterial strains) showed a significant decrease in toxicity of the laccase-treated ENR and FLU solution. © 2020 Global NEST.
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