Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Antibiotic-Associated Diarrhea Mediated by Toxigenic Clostridioides Difficile Infection in Iran: A Clinical Survey by Multiplex Real-Time Polymerase Chain Reaction and Toxigenic Culture



Khodaparast S1 ; Mobarez AM1 ; Khoramabadi N1 ; Vasei M2 ; Kamalabadifarahani M3 ; Saberifiroozi M4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  2. 2. Cell-based Therapies Research Center, Digestive Disease Research Institute, Shariati Hospital, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
  4. 4. Digestive Disease Research Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: Govaresh Published:2022

Abstract

Background: Clostridioides difficile is one of the important causes of hospital infections worldwide. Regarding the importance of Clostridioides difficile infection (CDI) epidemics and limited available prevalence reports of CDI in Iran, the present investigation was done on the incidence of CDI in hospitalized patients from 2017-2018. Our study outlined the requirements for CDI and followed up on the causative agent of antibiotic-associated diarrhea (AAD). Materials and Methods: We evaluated the CDI rates by multiplex real-time polymerase chain reaction (PCR) assay directly from inpatient fecal samples with a history of antibiotic therapy (2-8 weeks) and combined with anaerobic culture and toxicity assessments to isolate toxigenic and non-toxigenic types. The results were analyzed through one-way analysis of variance (ANOVA), pairwise two-tailed correlation, and regression using SPSS software version 25.0 (IBM® SPSS® Statistics, USA). Results: Among 491 fecal samples, 49 (9.9%) toxigenic C. difficile were characterized by real-time PCR, while 40 were isolated by toxigenic culture and cytotoxicity assay. Toxin profiling showed 43 (9.7%) tcdA+/tcdB+ and 6 (1.4%) tcdB+. Conclusion: A considerable prevalence of CDI among patients with AAD was demonstrated, and causative organisms mostly produce both toxins A and B. Since C. difficile still has problematic treatment and is costly, rapid and early detection may help to curb C. difficile infection more efficaciously. © 2022 Iranian Association of Gastroenterology and Hepatology. All rights reserved.
Related Docs
1. A Two-Step Approach for Diagnosing Glutamate Dehydrogenase Genes by Conventional Polymerase Chain Reaction From Clostridium Difficile Isolates, Middle East Journal of Digestive Diseases (2019)
2. Toxigenic Clostridium Difficile-Mediated Diarrhoea in Hematopoietic Stem Cell Transplantation In-Patients: Rapid Diagnosis and Efficient Treatment, Epidemiology and Infection (2021)
3. Identification of Cytotoxin-Producing Klebsiella Oxytoca Strains Isolated From Clinical Samples With Cell Culture Assays, Microbial Pathogenesis (2017)
Experts (# of related papers)
View all Related Experts
Mehdi Saberi Firoozi (2)
Mohammad Vasei (1)