Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Insight Into Immobilization Efficiency of Lipase Enzyme As a Biocatalyst on the Graphene Oxide for Adsorption of Azo Dyes From Industrial Wastewater Effluent Publisher



Yao LW1 ; Ahmed Khan FS1 ; Mubarak NM2 ; Karri RR2 ; Khalid M3 ; Walvekar R4 ; Abdullah EC5 ; Mazari SA6 ; Ahmad A7 ; Dehghani MH8, 9
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Chemical Engineering, Faculty of Engineering and Science, Curtin University, Miri Sarawak, 98009, Malaysia
  2. 2. Petroleum and Chemical Engineering, Faculty of Engineering, Universiti Teknologi Brunei, Bandar Seri Begawan BE1410, Brunei Darussalam
  3. 3. Graphene & Advanced 2D Materials Research Group (GAMRG), School of Engineering and Technology, Sunway University, No. 5, Jalan University, Bandar Sunway, Selangor, Subang Jaya, 47500, Malaysia
  4. 4. Department of Chemical Engineering, School of Energy and Chemical Engineering Xiamen University Malaysia, Jalan Sunsuria, Bandar Sunsuria, Selangor, Sepang, 43900, Malaysia
  5. 5. Malaysia-Japan International Instute of Technology, Universiti Teknologi Malaysia, Jalan Sultan Yahya Petra, Kuala Lumpur, 54100, Malaysia
  6. 6. Department of Chemical Engineering, Dawood University of Engineering and Technology, Karachi, 74800, Pakistan
  7. 7. Departamento de Quimica Organica, Universidad de Cordoba, Edificio Marie Curie (C-3), Ctra Nnal IV-A, Km 396, Cordoba, E14014, Spain
  8. 8. Department of Environmental Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  9. 9. Institute for Environmental Research, Center for Solid Waste Research, Tehran University of Medical Sciences, Tehran, Iran

Source: Journal of Molecular Liquids Published:2022


Abstract

Immobilization of enzymes improves their stability, performance, reusability, and recovery. This study investigated the removal of Azo dyes from industrial wastewater effluent using immobilized Lipase enzyme on Graphene Oxide (GO) in batch mode. The Lipase enzyme extracted from Porcine Pancreas was immobilized onto the GO via adsorption, where the enzymes are attached to the support by intermolecular forces. This study aims to investigate the immobilization efficiency of Lipase on the GO and determine the effect of parameters such as Lipase concentration, pH, and temperature on the enzyme activity. The results showed that the enzyme activity increased with the Lipase concentration, pH and temperature until an optimum point was achieved. The saturation of support surface pores causes the loss of enzyme activity due to the excessive Lipase enzyme, structural deformation of the support surface and enzyme denaturation due to extreme pH and temperature. The immobilized Lipase activity and free Lipase activity were compared. The optimum Lipase concentration is 6 mg/mL, with the greatest immobilization efficiency and highest enzyme activity. Besides, the optimum pH for the highest immobilized Lipase activity is 8.0, and the optimum temperature is 40 °C. The characterization results confirmed the immobilization of the Lipase enzyme. Adsorption isotherm and kinetic studies are also carried out to investigate the dye removal performances. The highest Azo dye removal efficiency obtained is 89.47% at 240 min of contact time and 5 mg/L of initial dye concentration. Further, the reusability of the immobilized Lipase was also investigated and found that the immobilized Lipase can be reused up to four cycles. Thus, the adsorption of dyes through immobilized lipase on GO can significantly impact various industrial sectors. © 2022
Related Docs
View other Related Docs
1. Biodegradation of Acid Orange-7 Dye by Immobilized Laccase on Functionalized Zsm-5 Zeolites: Investigation of the Role of Functionalization and Sio2/Al2o3 Ratio of Zeolite on the Catalytic Performance, Journal of Molecular Structure (2023)
2. Novel Insights Into Graphene Oxide-Based Adsorbents for Remediation of Hazardous Pollutants From Aqueous Solutions: A Comprehensive Review, Journal of Molecular Liquids (2023)
3. Role of Carbon Nanomaterials for Wastewater Treatment—A Brief Review, Water Treatment Using Engineered Carbon Nanotubes (2023)
4. Recent Advances on Sustainable Adsorbents for the Remediation of Noxious Pollutants From Water and Wastewater: A Critical Review, Arabian Journal of Chemistry (2023)
5. Regression and Mathematical Modeling of Fluoride Ion Adsorption From Contaminated Water Using a Magnetic Versatile Biomaterial & Chelating Agent: Insight on Production & Experimental Approaches, Mechanism and Effects of Potential Interferers, Journal of Molecular Liquids (2020)
6. Modeling and Experiment Study of Carbon Nanotubes for Enhancing the Oil Removal From Wastewater, Water Treatment Using Engineered Carbon Nanotubes (2023)
7. Enzyme Immobilization Onto the Nanomaterials: Application in Enzyme Stability and Prodrug-Activated Cancer Therapy, International Journal of Biological Macromolecules (2020)
8. Removal of Mercury From Industrial Effluents by Adsorption and Advanced Oxidation Processes: A Comprehensive Review, Journal of Molecular Liquids (2022)
Experts (# of related papers)
View all Related Experts
Mohammadhadi Dehghani Tafti (9)
Mohammad Ali Faramarzi (7)
Other Related Docs
9. Role of Hybrid Nanomaterials for a Sustainable Environment, Hybrid Nanomaterials for Sustainable Applications: Case Studies and Applications (2023)
10. Immobilization of Thermoalkalophilic Lipase From Bacillus Atrophaeus Fshm2 on Amine-Modified Graphene Oxide Nanostructures: Statistical Optimization and Its Application for Pentyl Valerate Synthesis, Applied Biochemistry and Biotechnology (2020)
11. Magnetic Casein Aggregates As an Innovative Support Platform for Laccase Immobilization and Bioremoval of Crystal Violet, International Journal of Biological Macromolecules (2022)
12. Fast Anisotropic Growth of the Biomineralized Zinc Phosphate Nanocrystals for a Facile and Instant Construction of Laccase@Zn3(Po4)2 Hybrid Nanoflowers, International Journal of Biological Macromolecules (2022)
13. Hierarchically-Structured Laccase@Ni3(Po4)2 Hybrid Nanoflowers for Antibiotic Degradation: Application in Real Wastewater Effluent and Toxicity Evaluation, International Journal of Biological Macromolecules (2023)
14. Novel Green Adsorbents for Removal of Aniline From Industrial Effluents: A Review, Journal of Molecular Liquids (2022)
15. Instantaneous Synthesis and Full Characterization of Organic–Inorganic Laccase-Cobalt Phosphate Hybrid Nanoflowers, Scientific Reports (2022)
16. Sustainable Remediation Technologies for Removal of Pesticides As Organic Micro-Pollutants From Water Environments: A Review, Applied Surface Science Advances (2024)
17. Optimization of Immobilization Conditions of Bacillus Atrophaeus Fshm2 Lipase on Maleic Copolymer Coated Amine-Modified Graphene Oxide Nanosheets and Its Application for Valeric Acid Esterification, International Journal of Biological Macromolecules (2020)