Phenotypic and Genotypic Characterization of Esbl-, Ampc-, and Carbapenemase-Producing Klebsiella Pneumoniae and Escherichia Coli Isolates

Phenotypic and Genotypic Characterization of Esbl-, Ampc-, and Carbapenemase-Producing Klebsiella Pneumoniae and Escherichia Coli Isolates Publisher Pubmed

Kazemian H^{1, 2} ; Heidari H³ ; Ghanavati R⁴ ; Ghafourian S¹ ; Yazdani F⁵ ; Sadeghifard N¹ ; Valadbeigi H¹ ; Maleki A¹ ; Pakzad I¹

Show Affiliations

1. Clinical Microbiology Research Center, Ilam University of Medical Sciences, Pajouhesh Blvd, Banganjab, Ilam, Iran
2. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Microbiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
4. Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
5. Molecular Microbiology Research Center, Faculty of Medicine, Shahed University, Tehran, Iran

Source: Medical Principles and Practice Published:2019

Abstract

Objectives: Drug resistance among gram-negative bacteria is a worldwide challenge. Due to the importance of drug-resistant Klebsiella pneumoniae and Escherichia coli strains in hospital-acquired infections, we aimed to determine the phenotypic and genotypic characteristics of ESBL-, AmpC-, and carbapenemase-producing isolates obtained from hospitalized patients in Tehran and Ilam (Iran). Materials and Methods: In total, 90 K. pneumoniae isolates and 65 E. coli isolates were collected from various infections. Phenotypic identification of bacterial isolates was performed using standard methods. Phenotypic screening of ESBL, AmpC, and carbapenemase enzymes was carried out. Detection of ESBL, AmpC, and carbapenemase genes was also performed by the PCR method. Results: Phenotypic detection tests showed that 36 (40%) K. pneumoniae and 23 (35.4%) E. coli isolates were ESBL producers. Moreover, 18 (20%) and 6 (9.2%) K. pneumoniae and E. coli isolates were AmpC producers, respectively. Modified Hodge test results indicated that 39 (43.3%) K. pneumoniae and 18 (27.7%) E. coli isolates produced carbapenemase. Molecular tests showed that 40% of K. pneumoniae and 36.9% of E. coli isolates were ESBL positive. AmpC was detected in 24.4 and 13.8% of K. pneumoniae and E. coli isolates. Carbapenemase was detected in 34 (37.8%) K. pneumoniae and 13 (20%) E. coli isolates. -Conclusion: In this study, 3 K. pneumoniae isolates simultaneously carried ESBL, AmpC, and carbapenemase genes. Up-to-date strategies such as combination therapy or utilization of new antimicrobial agents might help to combat such drug-resistant organisms. © 2019 The Author(s) Published by S. Karger AG, Basel.

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Style	Citing Format
MLA	Kazemian H, et al.. "Phenotypic and Genotypic Characterization of Esbl-, Ampc-, and Carbapenemase-Producing Klebsiella Pneumoniae and Escherichia Coli Isolates." Medical Principles and Practice, vol. 28, no. 6, 2019, pp. 547-551.
APA	Kazemian H, Heidari H, Ghanavati R, Ghafourian S, Yazdani F, Sadeghifard N, Valadbeigi H, Maleki A, Pakzad I (2019). Phenotypic and Genotypic Characterization of Esbl-, Ampc-, and Carbapenemase-Producing Klebsiella Pneumoniae and Escherichia Coli Isolates. Medical Principles and Practice, 28(6), 547-551.
Chicago	Kazemian H, Heidari H, Ghanavati R, Ghafourian S, Yazdani F, Sadeghifard N, Valadbeigi H, Maleki A, Pakzad I. "Phenotypic and Genotypic Characterization of Esbl-, Ampc-, and Carbapenemase-Producing Klebsiella Pneumoniae and Escherichia Coli Isolates." Medical Principles and Practice 28, no. 6 (2019): 547-551.
Harvard	Kazemian H et al. (2019) 'Phenotypic and Genotypic Characterization of Esbl-, Ampc-, and Carbapenemase-Producing Klebsiella Pneumoniae and Escherichia Coli Isolates', Medical Principles and Practice, 28(6), pp. 547-551.
Vancouver	Kazemian H, Heidari H, Ghanavati R, Ghafourian S, Yazdani F, Sadeghifard N, et al.. Phenotypic and Genotypic Characterization of Esbl-, Ampc-, and Carbapenemase-Producing Klebsiella Pneumoniae and Escherichia Coli Isolates. Medical Principles and Practice. 2019;28(6):547-551.
BibTex	@article{ author = {Kazemian H and Heidari H and Ghanavati R and Ghafourian S and Yazdani F and Sadeghifard N and Valadbeigi H and Maleki A and Pakzad I}, title = {Phenotypic and Genotypic Characterization of Esbl-, Ampc-, and Carbapenemase-Producing Klebsiella Pneumoniae and Escherichia Coli Isolates}, journal = {Medical Principles and Practice}, volume = {28}, number = {6}, pages = {547-551}, year = {2019} }
RIS	TY - JOUR AU - Kazemian H AU - Heidari H AU - Ghanavati R AU - Ghafourian S AU - Yazdani F AU - Sadeghifard N AU - Valadbeigi H AU - Maleki A AU - Pakzad I TI - Phenotypic and Genotypic Characterization of Esbl-, Ampc-, and Carbapenemase-Producing Klebsiella Pneumoniae and Escherichia Coli Isolates JO - Medical Principles and Practice VL - 28 IS - 6 SP - 547 EP - 551 PY - 2019 ER -

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