Characterization of Antimicrobial Resistance Patterns of Klebsiella Pneu-Moniae Isolates Obtained From Wound Infections Publisher Pubmed



Ghanavati R^{1, 2} ; Kazemian H^{3, 4, 5} ; Asadollahi P^{2, 3} ; Heidari H⁶ ; Irajian G² ; Navabmoghadam F² ; Razavi S² Show Affiliations
Source: Infectious Disorders - Drug Targets Published:2021

Abstract

Background: Multidrug resistance among ESBL producing isolates has limited the ad-ministration of proper antibiotics. It is, therefore, important to monitor the resistance patterns of Klebsiella pneumoniae isolates and provide infection control strategies to prevent nosocomial out-breaks. This study was aimed to determine antimicrobial resistance patterns of K. pneumoniae isolates obtained from wound infections of patients in Tehran, Iran. Methods: A total of 102 K. pneumoniae isolates were obtained from wound infections of patients in Tehran, Iran. The production of phenotypic ESBL and carbapenemase was assessed using the double-disc synergy test (DDST) and modified Hodge test (MHT), respectively. PCR was performed for the detection of ESBL, carbapenemase, quinolone and aminoglycoside resistance genes. Results: Forty-six (45.1%) and 23 (22.5%) isolates, out of the 102 isolates, were phenotypically detected as ESBL and carbapenemase producers, respectively. The PCR results showed that 80/102 (78.4%) and 51/102 (50%) isolates possessed at least one of the assessed ESBL and carbapene-mase genes, respectively. Quinolone resistance determinants (QRDs) and aac(6')-Ib genes were found amongst 50 (49%) and 67 (65.7%) isolates, respectively. Four isolates carried blaTEM, blaSHV, blaCTX-M, qnrB, qnrS and aac(6’)-Ib genes, simultaneously. Conclusion: Due to the presence of multiple resistance genes among some K. pneumoniae strains, antibiotic agents should be used with caution to preserve their efficacy in case of life-threatening in-fections. © 2021 Bentham Science Publishers.