Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Frequency of Aminoglycoside-Resistance Genes in Methicillin-Resistant Staphylococcus Aureus (Mrsa) Isolates From Hospitalized Patients Publisher



Mahdiyoun SM1 ; Kazemian H2 ; Ahanjan M3 ; Houri H4 ; Goudarzi M4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Microbiology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  2. 2. Department of Medical Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Microbiology, Molecular and Cellular Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  4. 4. Department of Medical Microbiology School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Jundishapur Journal of Microbiology Published:2016


Abstract

Background: Staphylococcus aureus is one of the most important causative agents in community- and hospital-acquired infections. Aminoglycosides are powerful bactericidal drugs that are often used in combination with beta-lactams or glycopeptides to treat staphylococcal infections. Objectives: The main objective of the present study was to determine the prevalence of aminoglycoside resistance among methicillin-resistant Staphylococcus aureus (MRSA) isolates in hospitalized patients in Sari and Tehran, Iran. Methods: In this study, 174 MRSA strains isolated from different clinical samples, such as blood, sputum, tracheal exudates, bronchus, pleura, urine, wounds, and catheters, were collected from hospitalized patients in Tehran and Sari during 2014. Antibiotic susceptibility testing was performed against nine antibiotics with the Kirby-Bauer disk diffusion method according to CLSI guidelines. The MRSA strains were examined with oxacillin and cefoxitin disks. MRSA was then validated by detection of the mecA gene. PCR was used to evaluate the prevalence of the aminoglycoside-resistance genes aac (6')-Ie/aph (2), aph (3')-IIIa, and ant (4') among the MRSA isolates. Results: The results of drug susceptibility testing showed that the highest rate of resistance was against erythromycin in Tehran (84.4%) and gentamicin (71.7%) in Sari. All isolates were sensitive to vancomycin, and all strains harbored the mecA gene. The aac (6')-Ie/aph (2), aph (3')-IIIa, and ant (4')-Ia genes were detected among 134 (77%), 119 (68.4%), and 122 (70.1%) of the isolates, respectively. Conclusions: The present study showed a high prevalence of aminoglycoside-resistance genes among MRSA isolates in two cities in Iran. © 2016. Ahvaz Jundishapur University of Medical Sciences.
Related Docs
1. Identification of Major Sequence Types Among Aminoglycoside Resistant Staphylococcus Aureus and Staphylococcus Epidermidis Strains Isolated From Clinical Samples, Iranian Journal of Microbiology (2022)
2. Frequency of the Genes Encoding Aminoglycoside Modifying Enzymes in Staphylococcus Aureus Isolated From Hospitalized Burn Patients, Journal of Mazandaran University of Medical Sciences (2016)
3. Prevalence of Genes Encoding Aminoglycoside-Modifying Enzymes in Clinical Isolates of Gram-Positive Cocci in Iran: A Systematic Review and Meta-Analysis, Microbial Drug Resistance (2020)
4. Methicillin-Resistant Staphylococcus Aureus (Mrsa) in Iran: A Systematic Review and Meta-Analysis, Journal of Global Antimicrobial Resistance (2018)
Experts (# of related papers)
View all Related Experts
Mohammad Emaneini (3)
Reza Beigverdi (2)