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Detection of Methicillin-Resistant Staphylococcus Aureus (Mrsa) in Clinical Samples of Patients With External Ocular Infection



Faridi A1 ; Tavakoli Kareshk A2 ; Fatahibafghi M3 ; Ziasistani M4 ; Kandehkar Ghahraman MR1 ; Seyyedyousefi SZ5 ; Shakeri N6 ; Kalantarneyestanaki D7
Authors
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Authors Affiliations
  1. 1. Student Research Committee, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
  2. 2. Infectious Diseases Reesearch Center, Birjand University of Medical Sciences, Birjand, Iran
  3. 3. Department of Microbiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences Yazd, Iran
  4. 4. Pathology and Stem Cell Research Center, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
  5. 5. Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Shahid Labafi Nejad Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  7. 7. Department of Microbiology and Virology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran

Source: Iranian Journal of Microbiology Published:2018

Abstract

Background and Objectives: Staphylococcus aureus is the main Gram-positive bacteria isolated from patients with ocular infections. Herein, we describe the pattern of antibiotic resistance, presence of resistance genes including ermA, ermB, ermC, msrA, mecA and the pvl cytotoxin gene in S. aureus isolates collected from patients with external ocular infection. Materials and Methods: In this study, 8 S. aureus isolates were collected from 81 patients that suffered from eye damage. Antibacterial susceptibility of isolates was determined using the Kirby-Bauer disk diffusion method. Resistance genes including ermA, ermB, ermC, msrA, mecA and the pvl virulence gene were detected by PCR method. Staphylococcal cassette chromosome mec (SCCmec) in MRSA isolates were detected by the multiplex-PCR method. Results: Three isolates were resistant to cefoxitin which is considered MRSA. The mecA gene was identified in MRSA isolates. SCCmec type IV and the pvl gene were detected in one of the MRSA isolates that was recovered from a diabetic patient. Conclusion: The emergence of S. aureus isolates belonging to SCCmec type IV and pvl gene among patients with ocular infection is very serious; therefore, identify genetic characterization of MRSA isolates for empirical therapy and infection control is very important. © 2018, Tehran University of Medical Science. All rights reserved.
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