Exploring Hsa_Circ_0100833 As a Potential Biomarker in Oral Squamous Cell Carcinoma: Bioinformatics and Experimental Insights Publisher Pubmed



Raei B ; Seyedena Y ; Hashemi M ; Hosseinkhan N ; Alaeddini M
Source: Clinical and Experimental Dental Research Published:2026

Abstract

Objectives: Oral squamous cell carcinoma (OSCC) is an aggressive malignancy with few reliable early diagnostic markers. Bioinformatic screening identified hsa_circ_0100833 as a differentially expressed circRNA in OSCC. Its potential significance in OSCC was then examined experimentally. Material and Methods: Differential circRNA expression was profiled using the GSE145608 dataset and Limma software. ROC curve analysis, circRNA–miRNA–mRNA interaction prediction, functional enrichment, and survival analysis were then used. Hsa_circ_0100833 expression was measured by qRT-PCR in 20 paired OSCC and adjacent normal tissues. This analysis was also performed in CAL-27 and FaDu cell lines (n = 3 each) and three normal human gingival fibroblast cultures. hsa-miR-607 and SORBS1 were quantified in parallel. Results: Differential expression analysis of the GSE145608 dataset identified hsa_circ_0100833 as down-regulated in OSCC. Bioinformatic analysis then predicted a binding interaction between this circRNA and hsa-miR-607. Hsa_circ_0100833 was significantly reduced in OSCC tissues and cell lines relative to normal controls; hsa-miR-607 was up-regulated, and SORBS1 showed parallel downregulation. ROC analysis revealed AUC values of 0.799 in cell lines and 0.712 in tissue samples. The predicted ceRNA network was enriched for PI3K-Akt signaling and focal adhesion pathways. Conclusion: hsa_circ_0100833 was down-regulated in OSCC and may act as a tumor suppressor through a hsa-miR-607/SORBS1-dependent mechanism. Its diagnostic potential warrants further investigation. © 2026 The Author(s). Clinical and Experimental Dental Research published by John Wiley & Sons Ltd.