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Up Regulation of Kai1 Gene Expression and Apoptosis Effect of Imatinib Mesylate in Gastric Adenocarcinoma (Ags) Cell Line Publisher



Shandiz SAS1 ; Farasati S2 ; Saeedi B2 ; Baghbaniarani F2 ; Asl EA3 ; Keshavarzpakseresht B2 ; Rahimi A4 ; Assadi A5 ; Noorbazargan H6 ; Hesari MR7 ; Mirzaie A1
Authors
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Authors Affiliations
  1. 1. Young Researchers and Elite Club, East Tehran Branch, Islamic Azad University, Tehran, Iran
  2. 2. Department of Genetics and Biotechnology, School of Biological Science, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran
  3. 3. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
  4. 4. Department of Virology, Pasteur Institute of Iran, Tehran, Iran
  5. 5. Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  7. 7. Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran

Source: Asian Pacific Journal of Tropical Disease Published:2016


Abstract

Objective: To evaluate the effect of imatinib mesylate on KAI1 gene expression and apoptosis properties in human gastric carcinoma AGS cell line. Methods: Cell viability was assessed by MTT assay and quantitative real time PCR method was applied for investigation of Bax, Bcl-2, and KAI1 gene expression in AGS cells. The quantity of KAI1, Bax, and Bcl-compared to GAPDH gene expressions were examined using the formula 2-δδCt. Furthermore, cell apoptosis/necrosis was carried out by annexin V/PI staining and quantified with flow cytometry after treatment with imatinib. Results: Imatinib mesylate was showed to have a dose-dependent toxicity effect against AGS cells. KAI1/GAPDH gene expression ratios were 1.07 ± 0.02 (> 0.05), 1.68 ± 0.19 (> 0.05), 3.60 ± 0.55 (< 0.05), 6.54 ± 0.27 (< 0.001) for 20, 50, 80 and 100 μmol/L of imatinib concentrations. The mRNA levels of Bax detected by real-time PCR after treatment with imatinib mesylate were significantly increased. Also, the number of apoptotic cells was increased from 3.72% (statistically significant; P < 0.05) in untreated AGS cells to 21.72%, 83.04% and 85.80%, respectively, following treatment with 20, 40, and 60 μmol/L imatinib mesylate. Conclusions: The results suggest that imatinib mesylate can induce apoptosis pathway in a dose-dependent mode and might modulate metastasis by up regulating KAI1 gene expression in human gastric carcinoma AGS cell line. © 2016 Asian Pacific Tropical Medicine Press.
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