Upregulation of Nm23, a Metastasis Suppressor Gene, in Human Prostate Adenocarcinoma (Pc-3) Cell Line Treated With Imatinib



Sadatshandiz SA¹ ; Shafieeardestani M² ; Irani S¹ ; Shahbazzadeh D³ Show Affiliations
Source: Journal of Isfahan Medical School Published:2015

Abstract

Background: Prostate cancer is one of the most common cancers diagnosed in developed countries. Many studies have confirmed that the nm23 gene suppresses metastasis in different types of cancers. The effects of, imatinib as the first member of tyrosine kinases inhibitors, were showed in research and treatment of solid tumors. The aim of the current study was to investigate the effect of imatinib on cell viability and suppressor metastasis nm23 gene expression in prostate cancer cell line. Methods: In this study, prostate cancer (PC-3) human cell line was treated with various concentrations of imatinib for 48 hours. Cell viability was assessed using MTT assay [3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide] and the half maximal inhibitory concentration (IC50) value was determined. We extracted RNA molecules via using RNX solution, after which cDNA was synthesized. The precise primers for the nm23 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes were designed via specific software. Then, the quantity of nm23 compared to GAPDH gene (reference gene) was analyzed using real-time polymerase chain reaction (PCR) method. Findings: Imatinib exerted an inhibitory effect on the viability of metastatic PC-3 cells. The calculated nm23/GAPDH gene expression ratio was 1.62 ± 0.02 (P < 0.01) in 21/33 mM concentration of imatinib at 48 hours. Conclusion: The results of this study showed that imatinib can inhibit metastasis via upregulating nm23 gene expression in prostate cancer adenocarcinoma PC-3 cell line. © 2015, Isfahan University of Medical Sciences(IUMS). All rights reserved.