The Clinical Significance of Vdr and Wifi Downregulation in Colorectal Cancer Tissue Publisher



Mir SM¹ ; Nezhadi A² ; Tehrani SS^{3, 4} ; Jamalpoor Z¹
Source: Gene Reports Published:2020

Abstract

Background: Activation of the Wnt/β catenin signaling pathway is the first event in the majority of colorectal cancers. Wnt inhibitory factor 1 (WIF1) inhibits the activity Wnt/β catenin pathway and prevents tumor growth through binding Wnt ligands. Vitamin D Receptor (VDR) modulates the transcription rate hundreds of target genes, specifically those involved in the inhibition of the Wnt/β catenin pathway. In this study, we aimed to analyze the expression of VDR and WIFI in patients with sporadic CRC. Methods: Our study population consisted of 47 sporadic CRC tissues and their neighboring non-cancerous tissues that were collected between 2006 and 2015. RNA extraction was performed by RNX-plus Total RNA Isolation Kit from tissues. After cDNA synthesis; the real-time PCR was performed to detect VDR and WIFI gene expression. Results: Our experiments revealed the mRNA expression levels of VDR and WIF1 were markedly lower in tumoral tissues in compared their marginal tissues, especially in the stage of III and stage IV (p-value <0.0001). In addition, there was statistically a positive correlation between VDR and WIFI expressions in tumoral tissues (r = 0.9329, p-value < 0.0001). Conclusion: Our findings indicated that expression levels of VDR and WIF1 was low in CRC tissues in comparison with marginal tissues. Thereby, VDR and WIFI may be considered as critical factors which may be associated with different stages of CRC, and it can be novel biomarkers for the diagnosis and prognosis of CRC, as well as can be a promising therapeutic target in CRC. © 2020