Production and Characterization of Single Chain Nimotuzumab: An in Vitro Study

Production and Characterization of Single Chain Nimotuzumab: An in Vitro Study Publisher

Pourafshar M¹ ; Safdari Y^{1, 2} ; Khajeniazi S² ; Yazdani Y³ ; Banisadr A¹ ; Veisi K⁴

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1. Department of Medical Biotechnology, Faculty of Advanced Technologies in Medicine, Golestan University of Medical Sciences, P.O. Box 4934174611, Gorgan, Iran
2. Cancer Research Center, Golestan University of Medical Sciences, Gorgan, Iran
3. Infection Disease Research Center, Golestan University of Medical Sciences, Gorgan, Iran
4. Biotechnology Department, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: International Journal of Peptide Research and Therapeutics Published:2018

Abstract

Recombinant expression and EGFR-binding activity assay of single chain nimotuzumab (nimotuzumab scFv) is reported in this study. The scFv was produced in VH-linker-VL format in Origami™ 2(DE3)pLysS bacterial cells and purified using Ni-TNA resin column. 3-D structure prediction using I-TASSER (Iterative Threading Assembly Refinement) server and analyzing the predicted models using YASARA (Yet Another Scientific Artificial Reality Application) viewer revealed that VH and VL domains assemble into a correctly-folded single chain antibody that is able to bind EGFR. The scFv was evaluated in ELISA and western blot tests and proven to be able to bind EGFR-overexpressing cancer cells (A-431 cells in an efficient manner but unable to recognize cancer cells expressing low levels of EGFR (MCF-7 breast cancer cells). © 2017, Springer Science+Business Media New York.

2. Use of Single-Chain Antibody Derivatives for Targeted Drug Delivery, Molecular Medicine (2016)

Style	Citing Format
MLA	Pourafshar M, et al.. "Production and Characterization of Single Chain Nimotuzumab: An in Vitro Study." International Journal of Peptide Research and Therapeutics, vol. 24, no. 1, 2018, pp. 171-178.
APA	Pourafshar M, Safdari Y, Khajeniazi S, Yazdani Y, Banisadr A, Veisi K (2018). Production and Characterization of Single Chain Nimotuzumab: An in Vitro Study. International Journal of Peptide Research and Therapeutics, 24(1), 171-178.
Chicago	Pourafshar M, Safdari Y, Khajeniazi S, Yazdani Y, Banisadr A, Veisi K. "Production and Characterization of Single Chain Nimotuzumab: An in Vitro Study." International Journal of Peptide Research and Therapeutics 24, no. 1 (2018): 171-178.
Harvard	Pourafshar M et al. (2018) 'Production and Characterization of Single Chain Nimotuzumab: An in Vitro Study', International Journal of Peptide Research and Therapeutics, 24(1), pp. 171-178.
Vancouver	Pourafshar M, Safdari Y, Khajeniazi S, Yazdani Y, Banisadr A, Veisi K. Production and Characterization of Single Chain Nimotuzumab: An in Vitro Study. International Journal of Peptide Research and Therapeutics. 2018;24(1):171-178.
BibTex	@article{ author = {Pourafshar M and Safdari Y and Khajeniazi S and Yazdani Y and Banisadr A and Veisi K}, title = {Production and Characterization of Single Chain Nimotuzumab: An in Vitro Study}, journal = {International Journal of Peptide Research and Therapeutics}, volume = {24}, number = {1}, pages = {171-178}, year = {2018} }
RIS	TY - JOUR AU - Pourafshar M AU - Safdari Y AU - Khajeniazi S AU - Yazdani Y AU - Banisadr A AU - Veisi K TI - Production and Characterization of Single Chain Nimotuzumab: An in Vitro Study JO - International Journal of Peptide Research and Therapeutics VL - 24 IS - 1 SP - 171 EP - 178 PY - 2018 ER -

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Abstract