Comparison of Restriction Enzyme Pattern Analysis and Full Gene Sequencing of 16S Rrna Gene for Nocardia Species Identification, the First Report of Nocardia Transvalensis Isolated of Sputum From Iran, and Review of the Literature

Comparison of Restriction Enzyme Pattern Analysis and Full Gene Sequencing of 16S Rrna Gene for Nocardia Species Identification, the First Report of Nocardia Transvalensis Isolated of Sputum From Iran, and Review of the Literature Publisher Pubmed

Fatahibafghi M^{1, 2} ; Heidarieh P³ ; Rasoulinasab M¹ ; Habibnia S¹ ; Hashemishahraki A⁴ ; Eshraghi SS¹

Show Affiliations

1. Department of Microbiology, Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Microbiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
3. Department of Bacteriology and Virology, Alborz University of Medical Sciences, Karaj, Iran
4. Department of Epidemiology, Pasteur Institute of Iran, Tehran, Iran

Source: Antonie van Leeuwenhoek# International Journal of General and Molecular Microbiology Published:2016

Abstract

Nocardial infections occur in different organs of the body and are common in immune disorder diseases of individuals. The aim of this study was to assess Nocardia species identification by phenotypic tests and molecular techniques applied to nocardiosis in Iranian patients. In the current study, various clinical samples were collected and cultured on conventional media and using the paraffin baiting method. Various phenotypic tests were performed. For accurate identification at the species level, restriction fragment length polymorphisms (RFLP) in the hsp65 and partial 16S rRNA genes and full gene sequencing of the 16S rRNA gene were used. Twenty-seven Nocardia spp. were isolated and analysis of phenotypic tests results showed Nocardia asteroides complex, Nocardia otitidiscaviarum, Nocardia nova, and Nocardia spp. New RFLP patterns of Nocardia strains with hsp65 and partial 16S rRNA genes were obtained. Full gene sequencing of the 16S rRNA gene identified Nocardia cyriacigeorgica, N. otitidiscaviarum, Nocardia farcinica, Nocardia transvalensis, and N. nova. Nocardia infections are rarely reported and this genus is the cause of various illnesses. Accurate identification of Nocardia spp. is important for epidemiology studies and treatment. It should also be noted that some species may have similar RFLP patterns; therefore, full gene sequencing of the 16S rRNA gene is necessary for confirmation. © 2016, Springer International Publishing Switzerland.

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Mohammad Mehdi Feizabadi (3)

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Style	Citing Format
MLA	Fatahibafghi M, et al.. "Comparison of Restriction Enzyme Pattern Analysis and Full Gene Sequencing of 16S Rrna Gene for Nocardia Species Identification, the First Report of Nocardia Transvalensis Isolated of Sputum From Iran, and Review of the Literature." Antonie van Leeuwenhoek# International Journal of General and Molecular Microbiology, vol. 109, no. 10, 2016, pp. 1285-1298.
APA	Fatahibafghi M, Heidarieh P, Rasoulinasab M, Habibnia S, Hashemishahraki A, Eshraghi SS (2016). Comparison of Restriction Enzyme Pattern Analysis and Full Gene Sequencing of 16S Rrna Gene for Nocardia Species Identification, the First Report of Nocardia Transvalensis Isolated of Sputum From Iran, and Review of the Literature. Antonie van Leeuwenhoek# International Journal of General and Molecular Microbiology, 109(10), 1285-1298.
Chicago	Fatahibafghi M, Heidarieh P, Rasoulinasab M, Habibnia S, Hashemishahraki A, Eshraghi SS. "Comparison of Restriction Enzyme Pattern Analysis and Full Gene Sequencing of 16S Rrna Gene for Nocardia Species Identification, the First Report of Nocardia Transvalensis Isolated of Sputum From Iran, and Review of the Literature." Antonie van Leeuwenhoek# International Journal of General and Molecular Microbiology 109, no. 10 (2016): 1285-1298.
Harvard	Fatahibafghi M et al. (2016) 'Comparison of Restriction Enzyme Pattern Analysis and Full Gene Sequencing of 16S Rrna Gene for Nocardia Species Identification, the First Report of Nocardia Transvalensis Isolated of Sputum From Iran, and Review of the Literature', Antonie van Leeuwenhoek# International Journal of General and Molecular Microbiology, 109(10), pp. 1285-1298.
Vancouver	Fatahibafghi M, Heidarieh P, Rasoulinasab M, Habibnia S, Hashemishahraki A, Eshraghi SS. Comparison of Restriction Enzyme Pattern Analysis and Full Gene Sequencing of 16S Rrna Gene for Nocardia Species Identification, the First Report of Nocardia Transvalensis Isolated of Sputum From Iran, and Review of the Literature. Antonie van Leeuwenhoek# International Journal of General and Molecular Microbiology. 2016;109(10):1285-1298.
BibTex	@article{ author = {Fatahibafghi M and Heidarieh P and Rasoulinasab M and Habibnia S and Hashemishahraki A and Eshraghi SS}, title = {Comparison of Restriction Enzyme Pattern Analysis and Full Gene Sequencing of 16S Rrna Gene for Nocardia Species Identification, the First Report of Nocardia Transvalensis Isolated of Sputum From Iran, and Review of the Literature}, journal = {Antonie van Leeuwenhoek# International Journal of General and Molecular Microbiology}, volume = {109}, number = {10}, pages = {1285-1298}, year = {2016} }
RIS	TY - JOUR AU - Fatahibafghi M AU - Heidarieh P AU - Rasoulinasab M AU - Habibnia S AU - Hashemishahraki A AU - Eshraghi SS TI - Comparison of Restriction Enzyme Pattern Analysis and Full Gene Sequencing of 16S Rrna Gene for Nocardia Species Identification, the First Report of Nocardia Transvalensis Isolated of Sputum From Iran, and Review of the Literature JO - Antonie van Leeuwenhoek# International Journal of General and Molecular Microbiology VL - 109 IS - 10 SP - 1285 EP - 1298 PY - 2016 ER -

Style

Citing Format

MLA

Fatahibafghi M, et al.. "Comparison of Restriction Enzyme Pattern Analysis and Full Gene Sequencing of 16S Rrna Gene for Nocardia Species Identification, the First Report of Nocardia Transvalensis Isolated of Sputum From Iran, and Review of the Literature." Antonie van Leeuwenhoek# International Journal of General and Molecular Microbiology, vol. 109, no. 10, 2016, pp. 1285-1298.

APA

Fatahibafghi M, Heidarieh P, Rasoulinasab M, Habibnia S, Hashemishahraki A, Eshraghi SS (2016). Comparison of Restriction Enzyme Pattern Analysis and Full Gene Sequencing of 16S Rrna Gene for Nocardia Species Identification, the First Report of Nocardia Transvalensis Isolated of Sputum From Iran, and Review of the Literature. Antonie van Leeuwenhoek# International Journal of General and Molecular Microbiology, 109(10), 1285-1298.

Chicago

Fatahibafghi M, Heidarieh P, Rasoulinasab M, Habibnia S, Hashemishahraki A, Eshraghi SS. "Comparison of Restriction Enzyme Pattern Analysis and Full Gene Sequencing of 16S Rrna Gene for Nocardia Species Identification, the First Report of Nocardia Transvalensis Isolated of Sputum From Iran, and Review of the Literature." Antonie van Leeuwenhoek# International Journal of General and Molecular Microbiology 109, no. 10 (2016): 1285-1298.

Harvard

Fatahibafghi M et al. (2016) 'Comparison of Restriction Enzyme Pattern Analysis and Full Gene Sequencing of 16S Rrna Gene for Nocardia Species Identification, the First Report of Nocardia Transvalensis Isolated of Sputum From Iran, and Review of the Literature', Antonie van Leeuwenhoek# International Journal of General and Molecular Microbiology, 109(10), pp. 1285-1298.

Vancouver

Fatahibafghi M, Heidarieh P, Rasoulinasab M, Habibnia S, Hashemishahraki A, Eshraghi SS. Comparison of Restriction Enzyme Pattern Analysis and Full Gene Sequencing of 16S Rrna Gene for Nocardia Species Identification, the First Report of Nocardia Transvalensis Isolated of Sputum From Iran, and Review of the Literature. Antonie van Leeuwenhoek# International Journal of General and Molecular Microbiology. 2016;109(10):1285-1298.

BibTex

@article{ author = {Fatahibafghi M and Heidarieh P and Rasoulinasab M and Habibnia S and Hashemishahraki A and Eshraghi SS}, title = {Comparison of Restriction Enzyme Pattern Analysis and Full Gene Sequencing of 16S Rrna Gene for Nocardia Species Identification, the First Report of Nocardia Transvalensis Isolated of Sputum From Iran, and Review of the Literature}, journal = {Antonie van Leeuwenhoek# International Journal of General and Molecular Microbiology}, volume = {109}, number = {10}, pages = {1285-1298}, year = {2016} }

RIS

TY - JOUR
AU - Fatahibafghi M
AU - Heidarieh P
AU - Rasoulinasab M
AU - Habibnia S
AU - Hashemishahraki A
AU - Eshraghi SS
TI - Comparison of Restriction Enzyme Pattern Analysis and Full Gene Sequencing of 16S Rrna Gene for Nocardia Species Identification, the First Report of Nocardia Transvalensis Isolated of Sputum From Iran, and Review of the Literature
JO - Antonie van Leeuwenhoek# International Journal of General and Molecular Microbiology
VL - 109
IS - 10
SP - 1285
EP - 1298
PY - 2016
ER -

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Abstract