The Efficacy of Commercial Tooth Storage Media for Maintaining the Viability of Human Periodontal Ligament Fibroblasts Publisher Pubmed



Lee W¹ ; Stover S¹ ; Rasoulianboroujeni M² ; Sherman K³ ; Fahimipour F^{2, 4} ; Dashtimoghadam E² ; Zito C² ; Jazayeri HE² ; Tayebi L²
Source: International Endodontic Journal Published:2018

Abstract

Aim: To evaluate Save-A-Tooth (SAT), EMT Toothsaver (EMT) and Hank's Balanced Salt Solution (HBSS) for their influence on the viability and proliferative capacity of human periodontal ligament fibroblasts (HPDLFs). Methodology: Primary HPDLFs were seeded into 96-well cell culture plates and exposed to SAT, EMT, HBSS and water (negative control) for 0.5, 1, 3, 6, 12 and 24 h at room temperature (22 °C). After each exposure time, cell viability was measured through quantifying adenosine triphosphate (ATP) using a luminescent dye. The proliferative capacity was also quantified using the PrestoBlue assay after 12 or 24 h storage in each medium. The data were analysed statistically by two-way anova and post hoc Least Significant Difference (LSD) test (P < 0.05). The morphology of the cells after 12 h storage was also investigated through live/dead viability/cytotoxicity kit together with fluorescence microscopy. Results: There was no significant difference in cell viability amongst HBSS, SAT and EMT groups up to 6 h. SAT was effective in maintaining cell viability only up to 12 h and then became detrimental to HPDLF; after 24 h, the effectiveness of SAT in maintaining cell viability was similar to that of water (P > 0.05). Amongst all the media, only EMT could maintain the proliferative capacity of HPDLFs significantly higher than the negative control, that is water (P < 0.05) after 24 h storage. Conclusion: EMT maintained the proliferative capacity of HPDLFs after 24 h storage. © 2017 International Endodontic Journal. Published by John Wiley & Sons Ltd