Co-Delivery of Il17rb Sirna and Doxorubicin by Chitosan-Based Nanoparticles for Enhanced Anticancer Efficacy in Breast Cancer Cells

Co-Delivery of Il17rb Sirna and Doxorubicin by Chitosan-Based Nanoparticles for Enhanced Anticancer Efficacy in Breast Cancer Cells Publisher Pubmed

Alinejad V^{1, 2} ; Hossein Somi M² ; Baradaran B¹ ; Akbarzadeh P³ ; Atyabi F⁴ ; Kazerooni H⁶ ; Samadi Kafil H⁵ ; Aghebati Maleki L¹ ; Siah Mansouri H¹ ; Yousefi M^{5, 7}

Show Affiliations

1. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2. Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
4. Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, 1714614411, Iran
5. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
6. Department of Biotechnology, Faculty of Chemical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran
7. Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

Source: Biomedicine and Pharmacotherapy Published:2016

Abstract

Overexpression of IL17RB is associated with poor prognosis and short survival of the breast cancer patients.IL17RB/IL17B signaling triggers a substantial increase in the cell growth, proliferation and migration through the activation of NF-κB as well as the up-regulation of the Bcl-2. In this study we designed carboxymethyl dextran (CMD) Chitosan nanoparticles (ChNPs) to encapsulated IL17RB siRNA and doxorubicin (DOX) as an anticancer drug. Then we investigated the efficiency of the simultaneous delivery of drug/siRNA on viability and gene expression of MDA-MB361 cell lines. Furthermore the efficacy of dual agent nanoparticles to induce apoptosis and inhibit migration of breast cancer cells was assessed by Annexin-V and wound healing assays respectively. Our results showed that DOX-siRNA-CMD-ChNPs had about 114 nm size; with polydispersity index and zeta potential about 0.3 and 10.1 mV respectively. Fourier transform infrared spectroscopy (FTIR) confirmed the formation of DOX-siRNA-CMD-ChNPs complex. In addition IL17RB siRNA had significant effect on DOX-induced cytotoxicity in MDA-MB361 cells. Furthermore treatment with dual agent nanoparticles resulted in a significant silencing of NF-κB and Bcl-2 relative gene expression, apoptosis induction and migration inhibition in MDA-MB361 cells. In conclusion, co-delivery of IL17RB siRNA and DOX can be considered as an effective system for the treatment of breast cancer. © 2016 Elsevier Masson SAS

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Style	Citing Format
MLA	Alinejad V, et al.. "Co-Delivery of Il17rb Sirna and Doxorubicin by Chitosan-Based Nanoparticles for Enhanced Anticancer Efficacy in Breast Cancer Cells." Biomedicine and Pharmacotherapy, vol. 83, no. , 2016, pp. 229-240.
APA	Alinejad V, Hossein Somi M, Baradaran B, Akbarzadeh P, Atyabi F, Kazerooni H, Samadi Kafil H, Aghebati Maleki L, Siah Mansouri H, Yousefi M (2016). Co-Delivery of Il17rb Sirna and Doxorubicin by Chitosan-Based Nanoparticles for Enhanced Anticancer Efficacy in Breast Cancer Cells. Biomedicine and Pharmacotherapy, 83(), 229-240.
Chicago	Alinejad V, Hossein Somi M, Baradaran B, Akbarzadeh P, Atyabi F, Kazerooni H, Samadi Kafil H, Aghebati Maleki L, Siah Mansouri H, Yousefi M. "Co-Delivery of Il17rb Sirna and Doxorubicin by Chitosan-Based Nanoparticles for Enhanced Anticancer Efficacy in Breast Cancer Cells." Biomedicine and Pharmacotherapy 83, no. (2016): 229-240.
Harvard	Alinejad V et al. (2016) 'Co-Delivery of Il17rb Sirna and Doxorubicin by Chitosan-Based Nanoparticles for Enhanced Anticancer Efficacy in Breast Cancer Cells', Biomedicine and Pharmacotherapy, 83(), pp. 229-240.
Vancouver	Alinejad V, Hossein Somi M, Baradaran B, Akbarzadeh P, Atyabi F, Kazerooni H, et al.. Co-Delivery of Il17rb Sirna and Doxorubicin by Chitosan-Based Nanoparticles for Enhanced Anticancer Efficacy in Breast Cancer Cells. Biomedicine and Pharmacotherapy. 2016;83():229-240.
BibTex	@article{ author = {Alinejad V and Hossein Somi M and Baradaran B and Akbarzadeh P and Atyabi F and Kazerooni H and Samadi Kafil H and Aghebati Maleki L and Siah Mansouri H and Yousefi M}, title = {Co-Delivery of Il17rb Sirna and Doxorubicin by Chitosan-Based Nanoparticles for Enhanced Anticancer Efficacy in Breast Cancer Cells}, journal = {Biomedicine and Pharmacotherapy}, volume = {83}, number = {}, pages = {229-240}, year = {2016} }
RIS	TY - JOUR AU - Alinejad V AU - Hossein Somi M AU - Baradaran B AU - Akbarzadeh P AU - Atyabi F AU - Kazerooni H AU - Samadi Kafil H AU - Aghebati Maleki L AU - Siah Mansouri H AU - Yousefi M TI - Co-Delivery of Il17rb Sirna and Doxorubicin by Chitosan-Based Nanoparticles for Enhanced Anticancer Efficacy in Breast Cancer Cells JO - Biomedicine and Pharmacotherapy VL - 83 IS - SP - 229 EP - 240 PY - 2016 ER -

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