Production and Characterization of Biosurfactant by Nocardia Species Isolated Form Soil Samples in Tehran Publisher



Javadi A¹ ; Pourmand MR¹ ; Hamedi J² ; Gharebaghi F¹ ; Baseri Z³ ; Rahdar HA⁴ ; Morovvati A¹ ; Mohammadzadeh R¹ ; Eshraghi SS¹ Show Affiliations
Source: Tenside# Surfactants# Detergents Published:2021

Abstract

In this work, we report the Nocardia species were initially screened and then tested for their ability to produce biosurfactant. The biosurfactant production from the Nocardia species was determined by qualitative standard methods. The selected bacterial strain had better oil spreading and emulsifying activity and surface tension reduction. The biosurfactant producer strain was identified by 16S rRNA gene sequencing method. Using Fourier transform infrared spectroscopy and comparative analysis, the biosurfactant structure could be detected. Oil spreading tests and blue agar plate tests confirmed the presence of biosurfactant and extracellular anionic glycolipids. The E24% assay using olive oil showed strong emulsifying characteristic of extracted biosurfactant with a 100%. The stability of the biosurfactant produced in different conditions was significant. Nocardia coubleae was identified by biochemical methods and molecular methods. © 2021 Walter de Gruyter GmbH, Berlin/Boston, Germany.