Isolation and Identification of Potentially Pathogenic Free-Living Amoeba in Drinking, Surface, and Stagnant Water Sources From Alborz Province, Iran

Isolation and Identification of Potentially Pathogenic Free-Living Amoeba in Drinking, Surface, and Stagnant Water Sources From Alborz Province, Iran Publisher Pubmed

Javanmard E¹ ; Niyyati M² ; Taghipour A³ ; Fatemi M² ; Mirjalali H⁴ ; Karanis P^{5, 6}

Show Affiliations

1. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3. Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran
4. Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5. Faculty of Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany
6. Department of Basic and Clinical Sciences, Nicosia University Medical School, Nicosia, 2408, Cyprus

Source: Journal of Water and Health Published:2022

Abstract

Free-living amoebas (FLAs) can cause neurological and ocular complications in humans. Water supplies play a critical role in transmitting FLAs to humans. The aim of the present study was to investigate the presence of FLAs in various aquatic sources including drinking water, stagnant water, and surface water in Alborz province, northern Iran, using morphological and molecular techniques. A total of 70 water samples were collected from 34 drinking waters, 23 surface waters, and 13 stagnant waters. Filtration and cultivation were employed to isolate FLAs. PCR assay was applied by using the genus-specific primers on positive samples. Pathogenicity tests (osmo- and thermo-tolerance properties) were performed for Acanthamoeba spp., positive sample. Considering the morphological criteria, four positive samples of Acanthamoeba sp., three Vermamoeba sp., two mixed Vermamoeba sp. with Vahlkamfiids, and one mixed Acanthamoeba sp. with Vahlkamfiids were isolated. Five Acanthamoeba sp. isolates were amplified using the JDP primer pairs. Among them, two genotypes, T4 (three isolates) and T5 (two isolates) corresponding to A. lenticulata, were identified. Four V. vermiformis samples were confirmed using the sequencing. This study highlighted the occurrence of potentially pathogenic waterborne FLAs in water habitats associated with high human activity. The results of such research on the prevalence of FLAs, as a human hazard, should be communicated to health policymakers. © 2022 The Authors

Related Docs

View other Related Docs

1. Isolation and Identification of Free-Living Amoeba From the Hot Springs and Beaches of the Caspian Sea, Parasite Epidemiology and Control (2020)

2. Molecular Identification of Pathogenic Free-Living Amoeba From Household Biofilm Samples in Iran: A Risk Factor for Acanthamoeba Keratitis, Microorganisms (2021)

3. Isolation, Identification, and Phylogenetic Analysis of Potentially Pathogenic Free-Living Amoebae Isolated From Nasal and Oral Mucosa of Hiv/Aids Patients in Iran, Parasitology Research (2019)

4. Molecular Identification of Acanthamoeba Genotypes Isolated From Oral Cavity of Heart Transplant Patients in Iran, Transplant Infectious Disease (2021)

5. Pathogenic Free-Living Amoebae Isolated From Contact Lenses of Keratitis Patients, Iranian Journal of Parasitology (2015)

6. Isolation and Genotyping of Acanthamoeba Strains (T4, T9, and T11) From Amoebic Keratitis Patients in Iran, Parasitology Research (2016)

7. Prevalence of Free-Living Amoebae in Five Rivers Associated With High Human Activity in Tehran Province, Iran, Journal of Water and Health (2025)

8. First Molecular Evidences of Acanthamoeba T3, T4 and T5 Genotypes in Hemodialysis Units in Iran, Acta Parasitologica (2019)

Experts (# of related papers)

View all Related Experts

Mahdi Mohebali (3)

Mohammad Soleimani (3)

Style	Citing Format
MLA	Javanmard E, et al.. "Isolation and Identification of Potentially Pathogenic Free-Living Amoeba in Drinking, Surface, and Stagnant Water Sources From Alborz Province, Iran." Journal of Water and Health, vol. 20, no. 4, 2022, pp. 620-629.
APA	Javanmard E, Niyyati M, Taghipour A, Fatemi M, Mirjalali H, Karanis P (2022). Isolation and Identification of Potentially Pathogenic Free-Living Amoeba in Drinking, Surface, and Stagnant Water Sources From Alborz Province, Iran. Journal of Water and Health, 20(4), 620-629.
Chicago	Javanmard E, Niyyati M, Taghipour A, Fatemi M, Mirjalali H, Karanis P. "Isolation and Identification of Potentially Pathogenic Free-Living Amoeba in Drinking, Surface, and Stagnant Water Sources From Alborz Province, Iran." Journal of Water and Health 20, no. 4 (2022): 620-629.
Harvard	Javanmard E et al. (2022) 'Isolation and Identification of Potentially Pathogenic Free-Living Amoeba in Drinking, Surface, and Stagnant Water Sources From Alborz Province, Iran', Journal of Water and Health, 20(4), pp. 620-629.
Vancouver	Javanmard E, Niyyati M, Taghipour A, Fatemi M, Mirjalali H, Karanis P. Isolation and Identification of Potentially Pathogenic Free-Living Amoeba in Drinking, Surface, and Stagnant Water Sources From Alborz Province, Iran. Journal of Water and Health. 2022;20(4):620-629.
BibTex	@article{ author = {Javanmard E and Niyyati M and Taghipour A and Fatemi M and Mirjalali H and Karanis P}, title = {Isolation and Identification of Potentially Pathogenic Free-Living Amoeba in Drinking, Surface, and Stagnant Water Sources From Alborz Province, Iran}, journal = {Journal of Water and Health}, volume = {20}, number = {4}, pages = {620-629}, year = {2022} }
RIS	TY - JOUR AU - Javanmard E AU - Niyyati M AU - Taghipour A AU - Fatemi M AU - Mirjalali H AU - Karanis P TI - Isolation and Identification of Potentially Pathogenic Free-Living Amoeba in Drinking, Surface, and Stagnant Water Sources From Alborz Province, Iran JO - Journal of Water and Health VL - 20 IS - 4 SP - 620 EP - 629 PY - 2022 ER -

Science Communicator Platform

Authors

Authors Affiliations

Abstract