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Antimicrobial Resistance, Prevalence of Resistance Genes, and Molecular Characterization in Intestinal Bacteroides Fragilis Group Isolates Publisher Pubmed



Kouhsari E1, 2 ; Mohammadzadeh N1 ; Kashanizadeh MG3 ; Saghafi MM4 ; Hallajzadeh M1 ; Fattahi A5 ; Ahmadi A2 ; Niknejad F2 ; Ghafouri Z6 ; Asadi A1 ; Boujary Nasrabadi MR1
Authors
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Authors Affiliations
  1. 1. Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran
  3. 3. Faculty of Medicine, Islamic Azad university, Tehran, Iran
  4. 4. Department of Clinical Pharmacology, Firouz Abadi Hospital, Iran University of Medical Sciences, Tehran, Iran
  5. 5. Center for Research and Training in Skin Disease and Leprosy, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Department of Biochemistry, Biophysics and Genetics, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

Source: APMIS Published:2019


Abstract

Since the level of antimicrobial resistance in Bacteroides fragilis has increased, monitoring the antimicrobial susceptibility could be necessary. The objectives of this study were to (i) investigate the prevalence of species, the occurrence of reduced antimicrobial susceptibility (E-test method), and antibiotic resistance genes in the B. fragilis group and (ii) evaluate the prevalence of enterotoxigenic B. fragilis and the distribution of bft gene subtypes in hospitalized patients. As many as 475 isolates out of 250 stool samples were detected to be B. fragilis group by using conventional biochemical tests (API-32A system) and multiplex-PCR. In addition, 48.2%, 13.9%, 76.6%, and 1.2% of B. fragilis group isolates were resistant (according to EUCAST breakpoint) to piperacillin-tazobactam, meropenem, clindamycin, and metronidazole, respectively. Six metronidazole-resistant strains were isolated; B. fragilis (n: 3), B. thetaiotaomicron, B. vulgates, and B. ovatus. The presence of the cfiA, cepA, ermF, and nim genes was observed in 3.8%, 15.9%, 34.1%, and 0.7% of the B. fragilis isolates, respectively. One hundred thirty-two B. fragilis isolates (27.8%)and 21 B fragilis isolates (15.9%) turned out to be bft gene positive by multiplex-PCR; eleven isolates (52.4%) harbored bft-1, eight isolates (38%) harbored bft-2 isotypes, and two isolates (9.5%) harbored bft-3 isotype (16.66%). These bacteria harbor antimicrobial resistance genes that could be transferred to other susceptible intestinal strains. Further investigations on lineage analysis are needed for a better understanding of these bacteria in Iran. © 2019 APMIS. Published by John Wiley & Sons Ltd
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